@article{Mayla Vasconcelos Adorno_Salina_Fernandes Joaquim_de Freitas Guimarães_Churocof Lopes_Menozzi_langoni_2021, place={Botucatu,}, title={PRESENÇA DE MOLLICUTES E MYCOPLASMA BOVIS EM SWABS NASAIS DE BEZERROS E NO LEITE DE VACAS COM MASTITE CLÍNICA}, volume={28}, url={https://rvz.emnuvens.com.br/rvz/article/view/520}, DOI={10.35172/rvz.2021.v28.520}, abstractNote={<p class="lista" align="justify"><span style="font-family: Times New Roman, serif;"><span lang="en-US"><em>Mycoplasma bovis </em></span></span><span style="font-family: Times New Roman, serif;"><span lang="en-US">is part of the bovine respiratory tract microbiota but is considered an opportunistic pathogen of extreme importance in respiratory diseases of calves. It causes to the herd several diseases such as mastitis, polyarthritis, pneumonia and endometritis. This pathogen is highly contagious and animals with mastitis are potential disseminators of infection to the herd since they release from 10</span></span><sup><span style="font-family: Times New Roman, serif;"><span lang="en-US">6</span></span></sup><span style="font-family: Times New Roman, serif;"><span lang="en-US"> to 10</span></span><sup><span style="font-family: Times New Roman, serif;"><span lang="en-US">8</span></span></sup><span style="font-family: Times New Roman, serif;"><span lang="en-US"> CFU per mL milk. Similarly, animals with pneumonia eliminate, through respiratory secretions, high microbial loads of the agent. The present study aimed to perform molecular detection of </span></span><span style="font-family: Times New Roman, serif;"><span lang="en-US"><em>Mycoplasma bovis</em></span></span><span style="font-family: Times New Roman, serif;"><span lang="en-US"> in 185 milk samples from cows with clinical mastitis, as well as in 50 nasal swab samples</span></span> <span style="font-family: Times New Roman, serif;"><span lang="en-US">from healthy calves with or without signs of pneumonia and born from cows with mastitis, all belonging to four dairy farms in Paraná State, where cases of mastitis had beendiagnosed. DNA extraction from both secretions was carried out according to the thermolysis method. For polymerase chain reaction (PCR), generic primers</span></span><span style="font-family: Times New Roman, serif;"><span lang="en-US"> were employed to amplify the </span></span><span style="font-family: Times New Roman, serif;"><span lang="en-US"><em>Mollicutes</em></span></span> <span style="font-family: Times New Roman, serif;"><span lang="en-US">DNA</span></span> <span style="font-family: Times New Roman, serif;"><span lang="en-US">and positive </span></span><span style="font-family: Times New Roman, serif;"><span lang="en-US">samples were subjected to PCR with primersspecific for </span></span><span style="font-family: Times New Roman, serif;"><span lang="en-US"><em>M.</em></span></span> <span style="font-family: Times New Roman, serif;"><span lang="en-US"><em>bovis</em></span></span><span style="font-family: Times New Roman, serif;"><span lang="en-US">. Positivity for </span></span><span style="font-family: Times New Roman, serif;"><span lang="en-US"><em>M. bovis</em></span></span><span style="font-family: Times New Roman, serif;"><span lang="en-US"> was 3.78% in milk samples, regardless of the farm, and 20% in nasal swabs.</span></span></p>}, journal={Veterinária e Zootecnia}, author={Mayla Vasconcelos Adorno, Brunna and Salina, Anelise and Fernandes Joaquim, Sâmea and de Freitas Guimarães, Felipe and Churocof Lopes, Bruna and Menozzi, Benedito and langoni, Helio}, year={2021}, month={mar.}, pages={1–9} }