CONSIDERAÇÕES SOBRE A OBTENÇÃO, PROCESSAMENTO, CARACTERIZAÇÃO E APLICAÇÃO TERAPÊUTICA DAS CÉLULAS-TRONCO MESENQUIMAIS EM MEDICINA EQUINA

Autores

  • Leandro Maia
  • Bruna De Vita
  • Carolina Nogueira de Moraes
  • Flavia Caroline Destro
  • Fernanda Cruz Landim-Alvarenga
  • Rogério Martins Amorim

Palavras-chave:

CTMs, cultivo, diferenciação, tratamento, cavalo

Resumo

Na medicina veterinária, o interesse nas células-tronco mesenquimais (CTMs) tem aumentando exponencialmente nos últimos anos, fato esse demonstrado pelo crescente número de pesquisas e publicações na área. Em equinos, a medula óssea e o tecido adiposo se destacam como as principais fontes para obtenção de CTMs para uso terapêutico, apresentando resultados na literatura bastante promissores, particularmente para o tratamento de lesões ortopédicas. Nesse sentido, o objetivo dessa revisão é descrever as características gerais das CTMs, relatando as fontes de obtenção, o processamento, a caracterização in vitro e aplicação terapêutica na medicina equina.

Referências

-Frisbie DD, Smith RKW, Clinical update on the use of mesenchymal stem cells in equine orthopaedics. Equi Vet Jou. 2010; 42:86-89.

-Brooke G, Cook M, Blair C, Han R, Heazlewood C, Jones B, et al. Therapeutic applications of mesenchymal stromal cells. Cell Dev Biol. 2007;18:846-858.

-Dahlgren LA. Stem Cell Therapy. In: Robinson NE, Sprayberry KA. Current Therapy in Equine Medicine. 6ª ed. St. Louis: SAUNDERS, 2009a, p. 908-911.

-Vidal MA, Robinson SO, Lopez MJ, Paulsen DB,Bor khsenious Johnson JR, Moore RM, et al. Comparison of chondrogenic potential in equine mesenchymal stromal cells derived from adipose tissue and bone marrow. Vet Surg. 2008; 37:713-724.

-Pereira LV. A importância do uso das células tronco para a saúde pública. Ciênc. saúde Coletiva. 2008;13:7-14.

-Martinello T, Bronzini I, Maccatrozoo L, Lacopetti I, Sampaolesi M, Mascarello F. et al. Cryopreservation Does Not Affect the Stem Characteristics of Multipotent Cells Isolated from Equine Peripheral Blood. Tissue Eng Part c Methods. 2010;16:771-781.

-Hoynowski SM, Fry MM, Gardner BM, Leming MT, Tucker JR, Black L. et al. Characterization and differentiation of equine umbilical cord-derived matrix cells. Biochem Biophysi Res Commun. 2007;362:347–353.

-Lovati A, Corradeti B, Lange CA, Recordati C, Bonacina E, Bizarro D. et al. Comparison of equine bone marrow-umbilical cord matrix and amniotic fluid-derived progenitor cells. Vet Res Commun. 2011;35:103-112.

-Nardi NB. Células-tronco: fatos, ficção e futuro. Genética na Escola. 2007. [cited 2011 nov 16]. Available from:

-Humpreys BD, Bonventre JV. Mesenchymal stem cells in acute injury kidney. Annu Rev Med. 2008;59:311-325.

-Chen Y, Shao JZ, Xiang LX, Dong XJ, Zhang GR. Mesenchymal stem cells: A promising candidate in regenerative medicine. Int J of Biochem Cell Biol. 2008a;40:815-820.

-Phinney DG, Prockop DJ. Concise review: mesenchymal stem/multipotent stromal cells: the state of transdifferentiation and modes of tissue repair—current views. Stem Cells. 2007;25:2896-2902.

-Chen L, Tredget EE, Wu PYG, Wu Y. Paracrine Factors of Mesenchymal Stem Cells Recruit Macrophages and Endothelial Lineage Cells and Enhance Wound Healing. Plos One. 2008b;3:1-12.

-Pacini S, Spinabella S, Trombi L, Galimberti S, Dini F, Carulicci F. Suspension of bone marrow-derived undifferentiae mesenchymal stromal cells for repair of superficial digital flexor tendon in race horses. Tissue Eng. 2007;12:2949-2955.

-Colleoni S, Bottani E, Tessaro I, Mari G, Merlo B, Romagnoli N, et al. Isolation, growth and differentiation of equine mesenchymal stem cells: effect of donor, source, amount of tissue and supplementation with basic fibroblast growth factor. Vet Res Commun. 2009;33:811-821.

-Violini S, Ramelli P, Pisani LF, Gorni C, Mariani P. Horse bone marrow mesenchymal stem cells express embryo stem cell markers and show the ability for tenogenic differentiation by in vitro exposure to BMP-12. BMC Cell Biol. 2009;10:1-10.

-Kasashima Y, Ueno T, Tomita A, Goodship AE, Smith RKW. Optimisation of bone marrow aspiration from the equine sternum for the safe recovery of mesenchymal stem cells. Equine Vet J. 2011;43:288-294.

-McLiwraith CW, Frisbie DD, Rodkey WG, Kisiday JD, Werpy NM, Kawcak CE. Evaluation of Intra-Articular Mesenchymal Stem Cells to Augment Healing of Microfractured Chondral Defects. Arthroscopic.2011;27:1552-1561.

-Koch TG, Heerckens T, Thomsen PD, Betts DH. Isolation of mesenchimal stem cells from equine umbilical cord blood. BMC Biotechnol. 2007;7:1-9.

-Reed SA, Jonhson SE. Equine umbilical cord blood contains a population of stem Cells that Express Oct4 and differentiate into mesodermal and endodermal cell types. J Cell Physiol. 2008;215:329–336.

-Koerner J, Nesic D, Romero JD, Brehm W, Mainil-varlet P, Grogan SP. Equine peripheral bood-derived progenitors in comparison to bone marrow-derived mesenchymal stem cells. Stem Cells. 2006;24:1613-1619.

-Mambelli LI, Santos EJC, Frazâo PJR.; Chaparro MB, Kerkis A, Zoppa ALV, et al. Characterization of Equine Adipose Tissue–Derived Progenitor Cells Before and After Cryopreservation. Tissue Eng Part c Methods. 2009;15:87-94.

-Latimer KS, Andrease CB. Bone Marrow. In: Cowell RL, Tyler RD. Diagnostic Cytology and Hematology of the Horse. 2ª ed. St. Louis: MOSBY,2002. p.217-226.

-Taylor SE, Clegg PD. Collection and Propagation Methods for Mesenchymal Stromal Cells. Vet Clin North Am Equine Pract. 2011;27:243–261.

-Maia L, Freitas NPP, De vita B, Landim-alvarenga FC, Amorim, RM. Avaliação do potencial osteogenico das células-tronco mesenquimais da medula óssea de equinos, isoladas com Ficoll-Paque de diferentes densidades. In: Anais do XII Conferência Anual da Abraveq; 2011ª, Campinas. Campinas: Associação Brasileira de Médicos Veterinários de Eqüídeos; 2011a, p.198-199.

-Latimer KS, Andrease CB. Bone Marrow. In: Cowell RL, Tyler RD. Diagnostic Cytology and Hematology of the Horse. 2ª ed. St. Louis: MOSBY, 2002 p.217-226.

-Fontes AM, Orellana MD, Prata KL. Células-tronco e seus métodos de estudo. In: Zago MA, Covas DT. Células-Tronco: a nova fronteira da medicina. São Paulo: ATHENEU, 2006. p.93-108.

-Radcliffe CH, Flaminio JBF, Fortier L. Temporal Analysis of Equine Bone Marrow Aspirate During Establishment of Putative Mesenchymal Progenitor Cell Populations. Stem Cells Dev. 2010;19:269-281.

-Toupadakis CA, Woung A, Genetos DC, Cheung WK, Borjesson DL, Leach JK, et al. Comparison of the osteogenic potential of equine mesenchymal stem cells from bone marrow, adipose tissue, umbilical cord blood, and umbilical cord tissue. Am J Vet Res. 2010;71:1237-1245.

-Dahlgren LA. Management of tendon injuries. In: Robinson NE, Sprayberry KA. Current Therapy in Equine Medicine. 6ª ed. St. Louis: SAUNDERS, 2009b. p. 518-523.

-Carvalho AM, Alves ALG, Oliveira PGG, Alvarez LEC, Laufer-amorim R, Hussni CA, et al. Use of Adipose Tissue-Derived Mesenchymal Stem Cells for Experimental Tendinitis Therapy in Equines. Journal Equine Veterinary Science. 2011;31:26-34.

-Vidal MA, Kilroy GE, Lopez MJ, Johnson JR, Moore RM, Gimble JM. Characterization of equine adipose tissue-derived stromal cells:adipogenic and osteogenic capacity and comparison with bone marrow-derived mesenchymal stromal cells. Vet Surg. 2007;36:613-622.

-Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini FC, Krauses DS, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy.2006;8:315-317.

-Arnhold SJ, Goletz I, Klein H, Stumpf G, Beluche LA, Rohde C, et al. Isolation and characterization of bone marrow–derived equine mesenchymal stem cells. Am J Vet Res. 2007;68:1095-1105.

-Guest DJ, Ousey JC, Smith MRW. Defining the expression of marker genes in equine mesenchymal stromal cells. Stem Cells and Cloning: Advances and Applications. 2008;1:1-9.

-Covas DT. Células-tronco Mesenquimais. In: Zago MA, Covas DT. Células-Tronco: a nova fronteira da medicina. São Paulo: ATHENEU, 2006ª. p.35-48.

-Borjesson DL, Peroni JF. The Regenerative Medicine Laboratory: Facilitating Stem Cell Therapy for Equine Disease. Clin Lab Med. 2011;31:109–123.

-Ibrahim S, Saudenrs K, Kydd JH, Lunn DP, Steinbach F. Screening of anti-human leukocyte monoclonal antibodies for reactivity with equine leukocyte. Vet Immun Immunopathol. 2007;119:63-80.

-Carvalho AM, Alves ALG, Golim MA, Moroz A, Hussni CA, Oliveira PGG, et al. Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue. Vet Immun Immunopathol. 2009;132:303-306.

-De Schauwer C, Meyer E, Van de Walle GR, Van Soom A. Markers of stemness in equine mesenchymal stem cells: a plea for uniformity. Theriogenology. 2010; p.1-13.

-Covas DT. A identificação das células-tronco. In: Zago MA, Covas DT. Células-Tronco: a nova fronteira da medicina. São Paulo: ATHENEU, 2006b. p.87-92.

-Müller M, Raabe O, Addicks K, Wenisch S, Arnhold S. Effects of non-steroidal anti-1 inflammatory drugs on proliferation, differentiation and migration in equine mesenchymal stem cells. Cell Biol Int. 2011;1:235-248.

-Stewart MC, Stewart AA. Mesenchymal Stem Cells: Characteristics, Sources, and Mechanisms of Action. Vet clin North Am Equine Pract. 2011;27:243–261.

-Barreira APB. Implante autólogo de células mesenquimais no tratamento de tendinites induzida em eqüinos: avaliação clínica, ultra-sonográfica e imunoistoquímica [Tese]. Botucatu: Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista; 2005.

-Crovace A, Lacitignola L, De Siena R, Rossi G, Francioso E. Cell therapy for tendon repairin horses: an experimental study. Vet Res Commun. 2007;31:281-283.

-Wilke MM, Nydam DV, Nixon AJ. Enhanced early chondrogenesis in articular defects following arthroscopic mesenchymal stem cell implantation in an equine model. J Orthop Res. 2007;25:913-925.

-Nixon AJ, Dahlgren LA, Haupt JL, Yeager AE, Ward DL. Effect of adipose-derived nucleated cell fractions on tendon repair in horseswith collagenase-induced tendinitis. Am J Vet Res. 2008;69:928-937.

-Maia L, Souza MV. Considerações sobre a tendinite do tendão do músculo flexor digital superficial de equinos. Revista Brasileira de Medicina Equina. 2009; 26:24-34.

-Smith RK, Korda M, Blunn GM, Goodship AE. Isolation and implantation of autologous equine mesenchymal stem cells from bone marrow into the superficial digital flexor tendon as a potential novel treatment. Equine Vet J. 2003;35:99-102.

-Lacitignola L, Crovace A, Rossi G, Francioso E. Cell therapy for tendinitis, experimental and clinical report. Vet Res Commun. 2008;32:33-38.

-Oliveira PGG. Implante autólogo de células mononucleares provenientes de medula óssea no tratamento de tendinites induzidas experimentalmente em eqüinos: avaliação a longo prazo – 120 dias [dissertação]. Botucatu: Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista; 2008.

-Del Blue M, Riccó S, Ramoni R, Conti V, Gnudi, G, Grolli S. Equine adipose-tissue derived mesenchymal stem cells and platelet concentrates: their association in vitro and in vivo. Vet Res Commun. 2008;32:51-55.

-Maia L, Amorim RM, Alvarenga FCL, Alvarenga ML, Joaquim JGF, Neto JFL, et al. Uso de la terapia celular en el tratamiento de parálisis del nervio facial: Reporte de caso. In II Congreso Latinoamericano de Neurologia Veterinária; 2009, Bogotá. Bogotá: Neurolatinvet; 2009. p.1-6.

-Marfe G, Massaro-Giordano M, Ranalli M, Cozzoli E, Di Stefano C, Malafoglia V. Blood derived stem cells: An ameliorative therapy in veterinary ophthalmology. J Cell Physiol. 2012; 227:1250-1256..

-Mambelli LI, Mattos RC, Winter G, Malschitzky E, Miglino MA, Kerkis I. Endometrosis treatment by equine adipose tissue-derived stem cells application. in: VI Congresso Brasileiro de Céluas-tronco e Terapia Celular; 2011, Salvador. Salvador: Associação Brasileira de Terapia Celular; 2011. p.133.

-Almeida FQ, Silva VP. Progresso científico em equideocultura na 1a década do século XXI. Revista Brasileira Zootecnia. 2010;39, supl. Especial:119-129.

Downloads

Publicado

2022-08-02

Como Citar

1.
Maia L, De Vita B, Moraes CN de, Destro FC, Landim-Alvarenga FC, Amorim RM. CONSIDERAÇÕES SOBRE A OBTENÇÃO, PROCESSAMENTO, CARACTERIZAÇÃO E APLICAÇÃO TERAPÊUTICA DAS CÉLULAS-TRONCO MESENQUIMAIS EM MEDICINA EQUINA. RVZ [Internet]. 2º de agosto de 2022 [citado 9º de outubro de 2024];20(3):359-77. Disponível em: https://rvz.emnuvens.com.br/rvz/article/view/1026

Edição

Seção

Artigos de Revisão

Artigos mais lidos pelo mesmo(s) autor(es)

1 2 > >>