Conteúdo lipídico, atividade mitocondrial e desenvolvimiento embrionário precoce de oócitos coletados de vacas mestiças (bos taurus indicus)
DOI:
https://doi.org/10.35172/rvz.2018.v25.27Palavras-chave:
Qualidade oocitária, conteúdo lipídico, predominância fenotípica, Bos indicus, Bos taurusResumo
O objetivo deste trabalho foi avaliar o efeito da predominância fenotípica no conteúdo lipídico como um indicador da qualidade do oócito. Os COC foram recuperados por aspiração folicular e submetidos a maturação in vitro (IVM), fertilização in vitro (FIV) e cultura in vitro (CIV). Determinou-se o conteúdo lipídico e atividade mitocondrial em oócitos imaturos e IVM. A taxa de maturação total era de 75%, com valores de 80,6% e 69,3% para oócitos predominantemente B. Indicus e predominantemente B. taurus, respectivamente. A taxa de fertilização total foi de 27,6%, para oócitos predominantemente B. indicus e predominantemente B. taurus, este foi de 26,1% e 29%, respectivamente. Um total de 52,1% de embriões divididos e 58,9% foi observada após 48 e 72 horas após a inseminação (hpi), respectivamente. Além disso, para o grupo predominantemente B. indicus 55,5% e 57,5% embrião clivada após 48 e 72 hpi foi observado. Enquanto para o grupo predominantemente B. taurus 52,1% e 58,9% dos embriões divididos após 48 e 72 hpi respectivamente. Oócitos imaturos mostraram gotas lipídicas mais pequenas (p<0,0001), em contraste com oócitos IVM com maior número de gotas lipídicas médias e grandes (p <0,0001). Oócitos predominantemente B. indicus mostraram mais gotas lipídicas pequenas (p = 0,0005) e médias (p = 0,005), enquanto que para as gotas lipídicas grandes não foram observadas diferenças significativas. Oócitos IVM tiveram maior atividade mitocondrial que o grupo de oócitos imaturos (p ˂ 0,05), não houve efeito de predominância fenotípica sobre este parâmetro. A avaliação do conteúdo lipídico não foi um preditor de qualidade dos oócitos nas vacas mestiças.
Referências
1. Kim J, Kinoshita M, Ohnishi M, Fukui Y. Lipid and fatty acid analysis of fresh and frozen–thawed immature and in vitro matured bovine oocyte. Reproduction. 2001;122:131-8.
2. Ferguson E, Leese H. A potential role for triglyceride as an energy source during bovine oocyte maturation and early embryo development. Mol Reprod Dev. 2006;73:1195-201.
3. Aardema H, Vos P, Lolicato F, Roelen B, Knijn H, Vaandrager A, Helms J, Gadella B. Oleic acid prevents detrimental effects of saturated fatty acids on bovine oocyte developmental competence. Biol Reprod. 2011;85: 62-9.
4. Krisher R. The effect of oocyte quality on development. J Anim Sci. 2004;82:E14-23.
5. Boni R. Origins and effects of oocyte quality in cattle. Anim Reprod. 2012;9:333-40.
6. Leroy J, Vanholder T, Mateusen B, Christophe A, Opsomer A, de Kruif A, et al. Nonesterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro. Reproduction. 2005;130:485-95.
7. Igosheva N, Abramov A, Poston L, Eckert J, Fleming T, Duchen M, et al. Maternal dietinduced obesity alters mitochondrial activity and redox status in mouse oocyte and zygote. PLoS One. 2010;5:e10074.
8. Wu L, Dunning K, Yang X, Russell D, Lane M, Norman R, et al. High-fat diet causes lipotoxicity responses in cumulus–oocyte complexes and decreased fertilization rates. Endocrinology. 2010;151:5438-45.
9. Grindler N, Moley K. Maternal obesity, infertility and mitochondrial dysfunction: potential mechanisms emerging from mouse model systems. Mol Hum Reprod. 2013;19:486-94. 10. Leroy J, Genicot G, Donnay I, Van Soom A. Evaluation of the lipid content in bovine oocytes and embryos with nile red: a practical approach. Reprod Domest Anim.2005;40:76-8.
11. Aranguren-Méndez J, Yáñez L. Planifique los cruzamientos. In: González-Stagnaro C, Soto-Belloso E. Manual de ganadería doble propósito. Maracaibo: Ediciones Astro Data SA; 2005. p.119-24.
12. Báez F, Chávez A, Hernández H, Villamediana P. Evaluación de la capacidad de desarrollo in vitro de ovocitos bovinos provenientes de vacas con predominancia fenotípica Bos taurus y Bos indicus. Rev Cient. 2010;20(3):259-67.
13. Isea-Villasmil W, Aranguren-Méndez J. Clasificación fenotípica en vacas mestizas. In: González-Stagnaro C, Belloso ES. Manual de ganadería doble propósito. Maracaibo: Ediciones Astro Data SA; 2005. p.76-81.
14. Sudano M, Paschoal D, Rascado T, Lima J, Landim-Alvarenga F. The effect of fetal calf serum concentrations upon the in vitro Bos taurus indicus x Bos taurus taurus crossbred embryo production and the cytoplasmic lipid accumulation. Vet Zootec. 2011;18(1):123-34.
15. Popescu L, Gherghiceanu M, Hinescu M, Cretoiu D, Ceafalan L, Regalia T, et al. Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC). J Cell Mol Med. 2006;10(2):429-58.
16. Ballard C, Looney C, Lindsey B, Pryor J, Lynn J, Bondioli K, et al. Comparing oocyte lipid content with circulant cholesterol and triglyceride levels of Bos taurus and Bos indicus donor cows. Reprod Fertil Dev. 2008;20:177.
17. Ordóñez-León E, Merchant H, Medrano A, Kjelland M, Romo S. Lipid droplet analysis using in vitro bovine oocytes and embryos. Reprod Domest Anim. 2014;49(2):306-14.
18. Hyttel P, Fair T, Callesen H, Greve T. Oocyte growth, capacitation and final maturation in cattle. Theriogenology. 1997;47(1):23-32.
19. Farese R, Walther T. Lipid droplets finally get a little R-E-S-P-E-C-T. Cell. 2009;139(5):855-60.
20. Yang H, Galea A, Sytnyk V, Crossley M. Controlling the size of lipid droplets: lipid and protein factors. Curr Opin Cell Biol. 2012;24(4):509-19.
21. Jeong W, Cho S, Lee H, Deb G, Lee Y, Kwon T, et al. Effect of cytoplasmic lipid content on in vitro developmental efficiency of bovine IVP embryos. Theriogenology. 2009;72(4):584-9.
22. Castaneda C, Kaye P, Pantaleon M, Phillips N, Norman S, Fry R, et al. Lipid content, active mitochondria and brilliant cresyl blue staining in bovine oocytes. Theriogenology. 2013;79(3):417-22.
23. Stojkovic M, Machado S, Stojkovic P, Zakhartchenko V, Hutzler P, Goncalves P, et al. Mitochondrial distribution and adenosine triphosphate content of bovine oocytes before and after in vitro maturation: correlation with morphological criteria and developmental capacity after in vitro fertilization and culture. Biol Reprod. 2001;64(3):904-9.
24. Nagano M, Katagiri S, Takahashi Y. ATP content and maturational/developmental ability of bovine oocytes with various cytoplasmic morphologies. Zygote. 2006;14(4):299-304.
25. Tarazona A, Rodríguez J, Restrepo L, Olivera-Ángel M. Mitochondrial activity, distribution and segregation in bovine oocytes and in embryos produced in vitro. Reprod Domest Anim. 2006;41(1):5-11.
26. Cerri R, Juchem S, Chebel R, Rutigliano H, Bruno R, Galvão K, et al. Effect of fat source differing in fatty acid profile on metabolic parameters, fertilization, and embryo quality in high-producing dairy cows. J Dairy Sci. 2009;92(4):1520-31.
27. Lopes A, Lane M, Thompson J. Oxygen consumption and ROS production are increased at the time of fertilization and cell cleavage in bovine zygotes. Hum Reprod. 2010;25(11):2762-73.
28. El Shourbagy S, Spikings E, Freitas M, John J. Mitochondria directly influence fertilization outcome in the pig. Reproduction. 2006;131:233-45.
29. Ge H, Tollner T, Hu Z, Dai M, Li X, Guan H, et al. The importance of mitochondrial metabolic activity and mitochondrial DNA replication during oocyte maturation in vitro on oocyte quality and subsequent embryo developmental competence. Mol Reprod Dev. 2012;79(6):392-401.
30. Camargo L, Viana J, Ramos A, Serapião R, de Sa W, Ferreira A, et al. Developmental competence and expression of the Hsp 70.1 gene in oocytes obtained from Bos indicus and Bos taurus dairy cows in a tropical environment. Theriogenology. 2007;68(4):626-32.
31. Paula-Lopes F, Chase C, Al-Katanani Y, Krininger C, Rivera R, Tekin S, et al. Genetic divergence in cellular resistance to heat shock in cattle: differences between breeds developed in temperate versus hot climates in responses of preimplantation embryos, reproductive tract tissues and lymphocytes to increased culture temperatures. Reproduction. 2003;125(2):285-94.
32. Hernández-Cerón J, Chase C, Hansen P. Differences in heat tolerance between preimplantation embryos from Brahman, Romosinuano, and Angus breeds. J Dairy Sci. 2004;87(1):53-8.
33. Chankitisakul V, Somfai T, Inaba Y, Techakumphu M, Nagai T. Supplementation of maturation medium with L-carnitine improves cryo-tolerance of bovine in vitro matured oocytes. Theriogenology. 2013;79(4):590-8.
34. Sutton-McDowall M, Feil D, Robker R, Thompson J, Dunning K. Utilization of endogenous fatty acid stores for energy production in bovine preimplantation embryos. Theriogenology. 2012;77(8):1632-41.
2. Ferguson E, Leese H. A potential role for triglyceride as an energy source during bovine oocyte maturation and early embryo development. Mol Reprod Dev. 2006;73:1195-201.
3. Aardema H, Vos P, Lolicato F, Roelen B, Knijn H, Vaandrager A, Helms J, Gadella B. Oleic acid prevents detrimental effects of saturated fatty acids on bovine oocyte developmental competence. Biol Reprod. 2011;85: 62-9.
4. Krisher R. The effect of oocyte quality on development. J Anim Sci. 2004;82:E14-23.
5. Boni R. Origins and effects of oocyte quality in cattle. Anim Reprod. 2012;9:333-40.
6. Leroy J, Vanholder T, Mateusen B, Christophe A, Opsomer A, de Kruif A, et al. Nonesterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro. Reproduction. 2005;130:485-95.
7. Igosheva N, Abramov A, Poston L, Eckert J, Fleming T, Duchen M, et al. Maternal dietinduced obesity alters mitochondrial activity and redox status in mouse oocyte and zygote. PLoS One. 2010;5:e10074.
8. Wu L, Dunning K, Yang X, Russell D, Lane M, Norman R, et al. High-fat diet causes lipotoxicity responses in cumulus–oocyte complexes and decreased fertilization rates. Endocrinology. 2010;151:5438-45.
9. Grindler N, Moley K. Maternal obesity, infertility and mitochondrial dysfunction: potential mechanisms emerging from mouse model systems. Mol Hum Reprod. 2013;19:486-94. 10. Leroy J, Genicot G, Donnay I, Van Soom A. Evaluation of the lipid content in bovine oocytes and embryos with nile red: a practical approach. Reprod Domest Anim.2005;40:76-8.
11. Aranguren-Méndez J, Yáñez L. Planifique los cruzamientos. In: González-Stagnaro C, Soto-Belloso E. Manual de ganadería doble propósito. Maracaibo: Ediciones Astro Data SA; 2005. p.119-24.
12. Báez F, Chávez A, Hernández H, Villamediana P. Evaluación de la capacidad de desarrollo in vitro de ovocitos bovinos provenientes de vacas con predominancia fenotípica Bos taurus y Bos indicus. Rev Cient. 2010;20(3):259-67.
13. Isea-Villasmil W, Aranguren-Méndez J. Clasificación fenotípica en vacas mestizas. In: González-Stagnaro C, Belloso ES. Manual de ganadería doble propósito. Maracaibo: Ediciones Astro Data SA; 2005. p.76-81.
14. Sudano M, Paschoal D, Rascado T, Lima J, Landim-Alvarenga F. The effect of fetal calf serum concentrations upon the in vitro Bos taurus indicus x Bos taurus taurus crossbred embryo production and the cytoplasmic lipid accumulation. Vet Zootec. 2011;18(1):123-34.
15. Popescu L, Gherghiceanu M, Hinescu M, Cretoiu D, Ceafalan L, Regalia T, et al. Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC). J Cell Mol Med. 2006;10(2):429-58.
16. Ballard C, Looney C, Lindsey B, Pryor J, Lynn J, Bondioli K, et al. Comparing oocyte lipid content with circulant cholesterol and triglyceride levels of Bos taurus and Bos indicus donor cows. Reprod Fertil Dev. 2008;20:177.
17. Ordóñez-León E, Merchant H, Medrano A, Kjelland M, Romo S. Lipid droplet analysis using in vitro bovine oocytes and embryos. Reprod Domest Anim. 2014;49(2):306-14.
18. Hyttel P, Fair T, Callesen H, Greve T. Oocyte growth, capacitation and final maturation in cattle. Theriogenology. 1997;47(1):23-32.
19. Farese R, Walther T. Lipid droplets finally get a little R-E-S-P-E-C-T. Cell. 2009;139(5):855-60.
20. Yang H, Galea A, Sytnyk V, Crossley M. Controlling the size of lipid droplets: lipid and protein factors. Curr Opin Cell Biol. 2012;24(4):509-19.
21. Jeong W, Cho S, Lee H, Deb G, Lee Y, Kwon T, et al. Effect of cytoplasmic lipid content on in vitro developmental efficiency of bovine IVP embryos. Theriogenology. 2009;72(4):584-9.
22. Castaneda C, Kaye P, Pantaleon M, Phillips N, Norman S, Fry R, et al. Lipid content, active mitochondria and brilliant cresyl blue staining in bovine oocytes. Theriogenology. 2013;79(3):417-22.
23. Stojkovic M, Machado S, Stojkovic P, Zakhartchenko V, Hutzler P, Goncalves P, et al. Mitochondrial distribution and adenosine triphosphate content of bovine oocytes before and after in vitro maturation: correlation with morphological criteria and developmental capacity after in vitro fertilization and culture. Biol Reprod. 2001;64(3):904-9.
24. Nagano M, Katagiri S, Takahashi Y. ATP content and maturational/developmental ability of bovine oocytes with various cytoplasmic morphologies. Zygote. 2006;14(4):299-304.
25. Tarazona A, Rodríguez J, Restrepo L, Olivera-Ángel M. Mitochondrial activity, distribution and segregation in bovine oocytes and in embryos produced in vitro. Reprod Domest Anim. 2006;41(1):5-11.
26. Cerri R, Juchem S, Chebel R, Rutigliano H, Bruno R, Galvão K, et al. Effect of fat source differing in fatty acid profile on metabolic parameters, fertilization, and embryo quality in high-producing dairy cows. J Dairy Sci. 2009;92(4):1520-31.
27. Lopes A, Lane M, Thompson J. Oxygen consumption and ROS production are increased at the time of fertilization and cell cleavage in bovine zygotes. Hum Reprod. 2010;25(11):2762-73.
28. El Shourbagy S, Spikings E, Freitas M, John J. Mitochondria directly influence fertilization outcome in the pig. Reproduction. 2006;131:233-45.
29. Ge H, Tollner T, Hu Z, Dai M, Li X, Guan H, et al. The importance of mitochondrial metabolic activity and mitochondrial DNA replication during oocyte maturation in vitro on oocyte quality and subsequent embryo developmental competence. Mol Reprod Dev. 2012;79(6):392-401.
30. Camargo L, Viana J, Ramos A, Serapião R, de Sa W, Ferreira A, et al. Developmental competence and expression of the Hsp 70.1 gene in oocytes obtained from Bos indicus and Bos taurus dairy cows in a tropical environment. Theriogenology. 2007;68(4):626-32.
31. Paula-Lopes F, Chase C, Al-Katanani Y, Krininger C, Rivera R, Tekin S, et al. Genetic divergence in cellular resistance to heat shock in cattle: differences between breeds developed in temperate versus hot climates in responses of preimplantation embryos, reproductive tract tissues and lymphocytes to increased culture temperatures. Reproduction. 2003;125(2):285-94.
32. Hernández-Cerón J, Chase C, Hansen P. Differences in heat tolerance between preimplantation embryos from Brahman, Romosinuano, and Angus breeds. J Dairy Sci. 2004;87(1):53-8.
33. Chankitisakul V, Somfai T, Inaba Y, Techakumphu M, Nagai T. Supplementation of maturation medium with L-carnitine improves cryo-tolerance of bovine in vitro matured oocytes. Theriogenology. 2013;79(4):590-8.
34. Sutton-McDowall M, Feil D, Robker R, Thompson J, Dunning K. Utilization of endogenous fatty acid stores for energy production in bovine preimplantation embryos. Theriogenology. 2012;77(8):1632-41.
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2018-12-04
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Mendez Y, Parra N, Baez F, Valeris R, Villamediana P. Conteúdo lipídico, atividade mitocondrial e desenvolvimiento embrionário precoce de oócitos coletados de vacas mestiças (bos taurus indicus). RVZ [Internet]. 4º de dezembro de 2018 [citado 21º de dezembro de 2024];25(1):120-31. Disponível em: https://rvz.emnuvens.com.br/rvz/article/view/27
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