Lipid content, mitochondrial activity and early embryo development in oocyte collected from crossbred cows (bos taurus indicus)
DOI:
https://doi.org/10.35172/rvz.2018.v25.27Keywords:
Oocyte quality, lipid content, phenotypic predominance, Bos indicus, Bos taurusAbstract
The objective of this research was to evaluate the effect of phenotypic predominance on lipid content, mitochondrial activity and early developmental competence as indicators of oocyte quality. Cumulus-oocyte complexes (COCs) were recovered through follicular aspiration, and underwent in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC) of presumptive zygotes. Lipid content and mitochondrial activity in immature and IVM oocytes were determined. A maturation rate of 80.6% and 69.3% was found for oocytes predominantly B. indicus and predominantly B. taurus, respectively. Total fertilization rate was 27.6%; 26.1% for predominantly B. indicus oocytes and 29% for predominantly B. taurus oocytes. A total of 55.5% and 57.5% of cleaved embryos after 48 and 72 h post-insemination (hpi) in predominantly B. indicus group were observed, respectively. As for the predominantly B. taurus group, 48.6% and 60.4% of cleaved embryos were found after 48 and 72 hpi, respectively. In both groups, immature oocytes showed a greater amount of small lipidic droplets (p <0.0001); IVM decreased the number of small lipid droplets (p < 0.0001) and increased the number of medium and large lipid droplets (p < 0.0001). Predominantly B. indicus oocytes had a greater number of small and medium-sized lipid droplets, while there were no significant differences in large lipid droplets. IVM oocytes had higher mitochondrial activity than immature oocytes group (p < 0.05) without any effect of phenotypic predominance on this parameter. Assessment of lipid content was not a predictive factor of oocyte quality in crossbred cows.
References
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34. Sutton-McDowall M, Feil D, Robker R, Thompson J, Dunning K. Utilization of endogenous fatty acid stores for energy production in bovine preimplantation embryos. Theriogenology. 2012;77(8):1632-41.
2. Ferguson E, Leese H. A potential role for triglyceride as an energy source during bovine oocyte maturation and early embryo development. Mol Reprod Dev. 2006;73:1195-201.
3. Aardema H, Vos P, Lolicato F, Roelen B, Knijn H, Vaandrager A, Helms J, Gadella B. Oleic acid prevents detrimental effects of saturated fatty acids on bovine oocyte developmental competence. Biol Reprod. 2011;85: 62-9.
4. Krisher R. The effect of oocyte quality on development. J Anim Sci. 2004;82:E14-23.
5. Boni R. Origins and effects of oocyte quality in cattle. Anim Reprod. 2012;9:333-40.
6. Leroy J, Vanholder T, Mateusen B, Christophe A, Opsomer A, de Kruif A, et al. Nonesterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro. Reproduction. 2005;130:485-95.
7. Igosheva N, Abramov A, Poston L, Eckert J, Fleming T, Duchen M, et al. Maternal dietinduced obesity alters mitochondrial activity and redox status in mouse oocyte and zygote. PLoS One. 2010;5:e10074.
8. Wu L, Dunning K, Yang X, Russell D, Lane M, Norman R, et al. High-fat diet causes lipotoxicity responses in cumulus–oocyte complexes and decreased fertilization rates. Endocrinology. 2010;151:5438-45.
9. Grindler N, Moley K. Maternal obesity, infertility and mitochondrial dysfunction: potential mechanisms emerging from mouse model systems. Mol Hum Reprod. 2013;19:486-94. 10. Leroy J, Genicot G, Donnay I, Van Soom A. Evaluation of the lipid content in bovine oocytes and embryos with nile red: a practical approach. Reprod Domest Anim.2005;40:76-8.
11. Aranguren-Méndez J, Yáñez L. Planifique los cruzamientos. In: González-Stagnaro C, Soto-Belloso E. Manual de ganadería doble propósito. Maracaibo: Ediciones Astro Data SA; 2005. p.119-24.
12. Báez F, Chávez A, Hernández H, Villamediana P. Evaluación de la capacidad de desarrollo in vitro de ovocitos bovinos provenientes de vacas con predominancia fenotípica Bos taurus y Bos indicus. Rev Cient. 2010;20(3):259-67.
13. Isea-Villasmil W, Aranguren-Méndez J. Clasificación fenotípica en vacas mestizas. In: González-Stagnaro C, Belloso ES. Manual de ganadería doble propósito. Maracaibo: Ediciones Astro Data SA; 2005. p.76-81.
14. Sudano M, Paschoal D, Rascado T, Lima J, Landim-Alvarenga F. The effect of fetal calf serum concentrations upon the in vitro Bos taurus indicus x Bos taurus taurus crossbred embryo production and the cytoplasmic lipid accumulation. Vet Zootec. 2011;18(1):123-34.
15. Popescu L, Gherghiceanu M, Hinescu M, Cretoiu D, Ceafalan L, Regalia T, et al. Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC). J Cell Mol Med. 2006;10(2):429-58.
16. Ballard C, Looney C, Lindsey B, Pryor J, Lynn J, Bondioli K, et al. Comparing oocyte lipid content with circulant cholesterol and triglyceride levels of Bos taurus and Bos indicus donor cows. Reprod Fertil Dev. 2008;20:177.
17. Ordóñez-León E, Merchant H, Medrano A, Kjelland M, Romo S. Lipid droplet analysis using in vitro bovine oocytes and embryos. Reprod Domest Anim. 2014;49(2):306-14.
18. Hyttel P, Fair T, Callesen H, Greve T. Oocyte growth, capacitation and final maturation in cattle. Theriogenology. 1997;47(1):23-32.
19. Farese R, Walther T. Lipid droplets finally get a little R-E-S-P-E-C-T. Cell. 2009;139(5):855-60.
20. Yang H, Galea A, Sytnyk V, Crossley M. Controlling the size of lipid droplets: lipid and protein factors. Curr Opin Cell Biol. 2012;24(4):509-19.
21. Jeong W, Cho S, Lee H, Deb G, Lee Y, Kwon T, et al. Effect of cytoplasmic lipid content on in vitro developmental efficiency of bovine IVP embryos. Theriogenology. 2009;72(4):584-9.
22. Castaneda C, Kaye P, Pantaleon M, Phillips N, Norman S, Fry R, et al. Lipid content, active mitochondria and brilliant cresyl blue staining in bovine oocytes. Theriogenology. 2013;79(3):417-22.
23. Stojkovic M, Machado S, Stojkovic P, Zakhartchenko V, Hutzler P, Goncalves P, et al. Mitochondrial distribution and adenosine triphosphate content of bovine oocytes before and after in vitro maturation: correlation with morphological criteria and developmental capacity after in vitro fertilization and culture. Biol Reprod. 2001;64(3):904-9.
24. Nagano M, Katagiri S, Takahashi Y. ATP content and maturational/developmental ability of bovine oocytes with various cytoplasmic morphologies. Zygote. 2006;14(4):299-304.
25. Tarazona A, Rodríguez J, Restrepo L, Olivera-Ángel M. Mitochondrial activity, distribution and segregation in bovine oocytes and in embryos produced in vitro. Reprod Domest Anim. 2006;41(1):5-11.
26. Cerri R, Juchem S, Chebel R, Rutigliano H, Bruno R, Galvão K, et al. Effect of fat source differing in fatty acid profile on metabolic parameters, fertilization, and embryo quality in high-producing dairy cows. J Dairy Sci. 2009;92(4):1520-31.
27. Lopes A, Lane M, Thompson J. Oxygen consumption and ROS production are increased at the time of fertilization and cell cleavage in bovine zygotes. Hum Reprod. 2010;25(11):2762-73.
28. El Shourbagy S, Spikings E, Freitas M, John J. Mitochondria directly influence fertilization outcome in the pig. Reproduction. 2006;131:233-45.
29. Ge H, Tollner T, Hu Z, Dai M, Li X, Guan H, et al. The importance of mitochondrial metabolic activity and mitochondrial DNA replication during oocyte maturation in vitro on oocyte quality and subsequent embryo developmental competence. Mol Reprod Dev. 2012;79(6):392-401.
30. Camargo L, Viana J, Ramos A, Serapião R, de Sa W, Ferreira A, et al. Developmental competence and expression of the Hsp 70.1 gene in oocytes obtained from Bos indicus and Bos taurus dairy cows in a tropical environment. Theriogenology. 2007;68(4):626-32.
31. Paula-Lopes F, Chase C, Al-Katanani Y, Krininger C, Rivera R, Tekin S, et al. Genetic divergence in cellular resistance to heat shock in cattle: differences between breeds developed in temperate versus hot climates in responses of preimplantation embryos, reproductive tract tissues and lymphocytes to increased culture temperatures. Reproduction. 2003;125(2):285-94.
32. Hernández-Cerón J, Chase C, Hansen P. Differences in heat tolerance between preimplantation embryos from Brahman, Romosinuano, and Angus breeds. J Dairy Sci. 2004;87(1):53-8.
33. Chankitisakul V, Somfai T, Inaba Y, Techakumphu M, Nagai T. Supplementation of maturation medium with L-carnitine improves cryo-tolerance of bovine in vitro matured oocytes. Theriogenology. 2013;79(4):590-8.
34. Sutton-McDowall M, Feil D, Robker R, Thompson J, Dunning K. Utilization of endogenous fatty acid stores for energy production in bovine preimplantation embryos. Theriogenology. 2012;77(8):1632-41.
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2018-12-04
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Mendez Y, Parra N, Baez F, Valeris R, Villamediana P. Lipid content, mitochondrial activity and early embryo development in oocyte collected from crossbred cows (bos taurus indicus). RVZ [Internet]. 2018 Dec. 4 [cited 2024 Jul. 3];25(1):120-31. Available from: https://rvz.emnuvens.com.br/rvz/article/view/27
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