Corynebacterium pseudotuberculosis in vitro Antimicrobial Sensitivity Profile of Samples Isolated from Sheeps and Goats with Caseous lymphadenitis from the Semiarid Regions of Pernambuco, Brazil

Abstract

The aim of the present study was to evaluate the in vitro sensitivity profile of 31 strains of Corynebacterium pseudotuberculosis isolated from sheeps and goats originated from the semi-arid regions of Pernambuco, Brazil. The contents of the abscesses were cultivated in sheep blood agar (10%) and the isolated colonies obtained were identified using Gram stain and API-Coryne biochemical identification kit (Bio-Merieux, France). All the strains were identified as Corynebacterium pseudotuberculosis biovar ovis. The sensitivity to antimicrobials were performed using the disk diffusion test. The results showed that 96.8% of the samples were sensitive to chloramphenicol and ciprofloxacin; 93.5% were sensitive to norfloxacin and cefazolin; 90.3% to amoxicillin and tetracycline; 87.1% to sulfa-trimethoprim; 83.9% to orbifloxacin; 77.4% to ampicillin, lincomycin and penicillin; 32% to gentamicin; 12.9% to novobiocin and 9.7% to neomycin. It was also noted that 87.1% of the samples were resistant to novobiocin; 80.6% to neomycin; 41.9% to gentamicin; 19.3% to lincomycin; 16.1% to ampicillin, orbifloxacin and penicillin; 12.9% to sulfa-trimethoprim; 6.4% to cefazolin, norfloxacin and amoxicillin, and 3.2% to ciprofloxacin, chloramphenicol and tetracycline. With the exception of one sample, all others showed some degree of multiple resistance, with 16.0% of them being multi-resistent to approximately 50.0% of the antimicrobial drugs tested.