Rabies virus monitoring in different species of bats in Maringá, southern Brazil

Authors

  • Alexandre Luiz Polizel
  • Helena Beatriz de Carvalho Ruthner Batis
  • Henrique Ortêncio Filho

DOI:

https://doi.org/10.35172/rvz.2017.v24.298

Keywords:

chiroptera, rabies vírus, saliva

Abstract

Rabies is an zoonotic infectious disease, caused by rabies virus (RABV). The bats with canids
are the main RABV reservoirs, accounting respectively for maintaining the air cycles and
terrestrial of the disease. This study aimed to identify interactions between the virus and the
reservoir in air rabies cycle. For this, the presence of RABV was investigated in bat saliva
samples from different species. Thirty-six capture bats were held in Maringá Region Parana,
southern Brazil, from April to December 2013. The bats were captured with the help of nylon
nets and placed in cotton bags. Were registered biometric data and collected a sample of oral
swab of each especimen. For the identification of RABV, the semi-nested RT-PCR technique
("Reverse transcription polymerase chain reaction") targeting the N gene encodes the
nucleoprotein of the virus was performed. Data analysis was performed using descriptive
statistics. Throughout the study, were captured 444 bats belonging to four families and fifteen
species. The RABV was not identified in the evaluated samples. These results demonstrate
the absence of excretion of RABV in the saliva of healthy bats in the studied area and
reinforce the need for more studies about the maintenance of rabies in different species of
bats.

References

1. International Committee on Taxonomy of Viruses. The Universal Virus Database, version
4 [Internet]. Ithaca, New York. 2016 [cited 2016 Mar 23]. Available
from://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/
2. Almeida MF, Aguiar EAC, Martorelli LFA, Silva MMS. Diagnóstico laboratorial de raiva
em quirópteros realizado em área metropolitana na região sudeste do Brasil. Rev Saude
Publica. 1994;28(5):341-4.
3. Silva LHQ, Cunha EMSC, Pedro WA, Cardoso TC, Souza MCC, Ferrari CIL. Isolamento
do vírus rábico em Molossus ater (Chiroptera: Molossidae) no Estado de São Paulo. Rev
Saude Publica. 1999;33(6):626-8.
4. Sodré MM, Gama AR, Almeida MF. Updated list of bat species positive for rabies in
Brazil. Rev Inst Med Trop São Paulo. 2010;52:75-81.
5. Batista HBCR, Franco AC, Roeche PM. Raiva: uma breve revisão. Acta Sci Vet.
2007;35(2):125-44.
6. Martorelli LFA, Aguiar EAC, Almeida MF, Silva MMS, Nunes VFP. Isolamento do vírus
rábico de morcego insetívoro, Lasiurus borealis. Rev Saude Publica. 1996;30(1):101-2
7. Silva MMS, Harman I, Gonçalves EFB, Uieda V. Bats from the metropolitan region of São
Paulo, southeastern Brazil. Chiropt Neotrop. 1996;2(1):39-41.
8. Reis NR, Lima IP, Peracchi AL. Morcegos (Chiroptera) da área urbana de Londrina,
Paraná, Brasil. Rev Bras Zool. 2006;19(3):739-46.
9. Mikich SB. A dieta dos morcegos frugívoros (Mammalia, Chiroptera, Phyllostomidae) de
um pequeno remanescente de floresta estacional semidecidual do sul do Brasil. Rev Bras
Zool. 2002;19(1):239-49.
10. Grenhall AM, Paradiso JL. Bats and bat banding. Washington: Bureau of Sport Fisheries
and Wild; 1968.
11. Vizotto LD, Taddei VA. Chave para determinação de quirópteros brasileiros. Bol Cienc.
1973;1:1-72.
12. Jones Jr JK, Carter DC. Annotated checklist, with keys to subfamilies and genera. In:
Baker RJ, Jones Jr JK, Carter DC. Special Publications Museum Texas Tech. University.
Biology of bats of the new world family Phyllostomidae. Part I. Lubbock: Texas Tech
Press; 1976. v.10, p.7-38.
13. Gregorin R, Taddei VA. Chave artificial para identificação de molossídeos brasileiros
(Mammalia, Chiroptera). Mastozool Neotrop. 2002;1(9):13-32.
14. Carnieli JRP, Fahl WO, Castilho JG, Oliveira RN, Macedo CI, Durymanova E, et al.
Characterization of Rabies virus isolated from canids and identification of the main wild
canid host in Northeastern Brazil. Virus Res. 2008;131:33-46.
15. Scheffer KC, Carrieri ML, Albas A, Santos HCP, Kotait I, Ito FH. Vírus da raiva em
quirópteros naturalmente infectados no Estado de São Paulo, Brasil. Rev Saude Publica.
2007;41(3):389-95.
16. World Health Organization. Expert Committee on Rabies, 8th Report. Geneve: WHO;
1992. (Technical report series, nº 824).
17. Harris SL, Aegerter JN, Brookes SM, Mcelhinney LM, Jones G, Smith GC, et al.
Targeted surveillance for european bat Lyssaviruses in english bats (2003-06). J Wildl
Dis. 2009;45(4):1030-41.
18. Schatz J, Ohlendorf B, Busse P, Pelz G, Dolch D, Theubner J, et al. Twenty years of
active bat rabies surveillance in Germany: a detailed analysis and future perspectives.
Epidemiol Infect. 2014;142(6):1155-66.
19. Echevarria JE, Avellón A, Juste J, Vera M, Ibánez C. Screening of active Lyssavirus
infection in wild bat populations by viral RNA detection on oropharyngeal swabs. J Clin.
2001;10(39):3678-83.

Published

2017-09-29

How to Cite

1.
Polizel AL, de Carvalho Ruthner Batis HB, Filho HO. Rabies virus monitoring in different species of bats in Maringá, southern Brazil. RVZ [Internet]. 2017 Sep. 29 [cited 2024 Jul. 22];24(3):613-9. Available from: https://rvz.emnuvens.com.br/rvz/article/view/298

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Section

Original Articles