Crotalus durissus cascavella VENOM TOXICITY TO MAMMALIAN CELLS

Authors

  • Lucelina da Silva Araújo
  • Danilo Damasceno Rocha
  • Daniel Araújo Viana
  • João Alison de Moraes Silveira
  • Francisco Sérgio Lopes Vasconcelos-Filho
  • Diego Veras Wilke
  • Diva Maria Borges- Nojosa
  • Claudia do O’ Pessoa
  • Manoel Odorico de Moraes
  • Janaina Serra Azul Monteiro Evangelista

Keywords:

apoptosis, Crotalus durissus cascavella, cytotoxicity, tumor cells, snake venom

Abstract

Snakes from the genus Crotalus gained significant space in the scientific field since the
development of researches with their venom involving cytotoxic activity. Crotalus durissus
cascavella is the only subspecies recorded of the Caatinga biome of Northeastern Brazil and
it’s noticed a lack of studies on its venom properties. The present study evaluated the crude
venom of C. d. cascavella for its cytotoxic activity in vitro against both tumor cell lines and
normal cells. The mechanism of cell death induced by this venom was also investigated. C. d.
cascavella venom presented high cytotoxicity (IC50 values ranging from 2.7 to 6.9 µg/mL)
against five tumor cells lines: OVCAR-8 and SKOV3 (ovarian carcinomas), PC-3M
(metastatic prostate carcinoma), MCF-7 (breast carcinoma), and SF-268 (glioblastoma) and
PBMC as well. The cells treated with C. d. cascavella venom triggered the apoptotic pathway
as confirmed by several methods. These results underline the biomedical potential of toxins
from the venom from this species.

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Published

2022-03-07

How to Cite

1.
Araújo L da S, Rocha DD, Viana DA, Silveira JA de M, Vasconcelos-Filho FSL, Wilke DV, Nojosa DMB-, O’ Pessoa C do, Moraes MO de, Evangelista JSAM. Crotalus durissus cascavella VENOM TOXICITY TO MAMMALIAN CELLS. RVZ [Internet]. 2022 Mar. 7 [cited 2024 Nov. 21];23(3):465-7. Available from: https://rvz.emnuvens.com.br/rvz/article/view/821

Issue

Vol. 23 No. 3 (2016)

Section

Original Articles

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