IDENTIFICACIÓN MORFOLÓGICA Y MOLECULAR DE METACERCARIAE DEL TREMATODO ZOONÓTICO (Haplorchis taitui) EN PECES DE AGUA DULCE EN IRÁN
DOI:
https://doi.org/10.35172/rvz.2021.v28.442Palabras clave:
Trematoda, Heterophyidae, Río Shapour, PCR.Resumen
El trematodo intestinal, Haplorchis taichui (Nishigori, 1924), es un elemento importante en la salud pública que causa infección en humanos y animales, especialmente en Asia y en partes de África y América. Se encontraron Haplorchis taitui metacercariae en las branquias de Cyprinion macrostomus (Heckel) y Capoeta barroisi persica (Karaman) recolectadas del río Shapour. Las metacercarias morfológicas extirpadas se identificaron como monturas húmedas bajo un microscopio estereoscópico. Luego, las muestras fueron sometidas a análisis molecular. El resultado mostró que el 69% de los peces examinados (n = 30) fueron diagnosticados infectados con metacercarias enquistadas en las branquias. La intensidad media fue de 8.3 ± 16.9 parásitos por pez. Los valores morfométricos concuerdan con los hallazgos de otros estudios con las pequeñas diferencias y la longitud del producto de reacción en cadena de la polimerasa y el análisis de la secuencia de nucleótidos del gen del ácido desoxirribonucleico ribosómico 18S mostró una similitud de más del 99% entre las muestras y el Haplorchis taichui (Nishigori, 1924) registrado en GenBank.
Citas
1. Africa CM, de Leon W, Garcia EY. Visceral complications in intestinal heterophyidiasis of man. Acta Med Philippina, Monographic Series. 1940; 1: 1-132.
2.Amaya-Huerta D, Almeyda-Artigas J. Confirmation of Centrocestus formosanus (Nishigori, 1924) Price, 1932 (Trematoda: Heterophyidae) in Mexico. Res. Rev. Parasitol. 1994; 54: 99-103.
3. Anderson GR. Identification and maturation of the metacercaria of Indodidymozoon pearsoni. J. Helminthol. 1999; 73:21–26.
4. Bartoli P, Jousson O, Russell-Pinto F. The life cycle of Monorchis parvus (Digenea: Monorchiidae) demonstrated by developmental and molecular data. J. Parasitol. 2000; 86: 479–489.
5.Belizario VY, Jr de Leon WU, Bersabe MJ. A focus of human infection by Haplorchis taichui (Trematoda: Heterophyidae) in the southern Philippines. J. Parasitol. 2004; 90: 1165-1169.
6. Boonmekam D, Namchote S, Nak-ai W, Glaubrecht M, Krailas D. The Prevalence of Human Intestinal Fluke Infections, Haplorchis taichui, in Thiarid Snails and Cyprinid Fish in Bo Kluea District and Pua District, Nan Province, Thailand. Silpakorn Uni. Science and Technol. J. 2016 ; 10:29-37
7. Bray RA, Gibson D, Jones A. Keys to the Trematoda. London: CAB International and Natural History Museum v. 3. 2008.
8. Chai JY, Murrell KD, Lambery AJ. Fish-borne parasitic zoonoses: status and issues. Int. J. Parasitol. 2005; 35: 1233-1254.
9.Chai JY. Intestinal flukes. In Murrell KD, Fried B eds, Food-borne Parasitic Zoonoses: Fish and Plant-borne Parasites. World Class Parasites, Vol. 11. New York, USA, Springer. 2007 ; 53-115.
10. Cribb TH, Anderson GR, Adlard RD, Bray RA. A DNAbased demonstration of a three host life-cycle for the Bivesiculidae (Platyhelminthes: Digenea). Inter. J. Parasitol. 1998; 28:1791–1795.
11. Dung DT, De NV, Waikagul J. Fishborne zoonotic intestinal trematodes. Emerg. Infect. Dis. j. 2007; 13: 1828–1833.
12.Golchin Manshadi AR, Mirghafari S. Composition and distribution of macrobenthic invertebrates of Shapour River, Fars south-west, Iran. IJBPAS. 2015; 4: 5004-5012.
13. Hillis DM, Dixon, MT. Ribosomal DNA: molecular evolution and phylogenetic inference. Quart. Rev. Biol. 1991; 66:411–453.
14. Jousson O, Bartoli P, Zaninetti L, Pawlowski J. Use of the ITS rDNA for elucidation of some life cycles of mesometridae (Trematoda, Digenea). Inter. J. Parasitol. 1998; 28:1403–1411.
15. Levy MG, Flowers JR, Poore MF, Mullen JE, Khoo L, Pote LM, et al. Morphologic, pathologic, and genetic investigations of Bolbophorus spp. (Diplostomatida, Trematoda) affecting cultured Ictalurus punctatus in the Mississippi Delta. J. Aqu. Animal Health. 2002; 14:235–246.
16. Littlewood DTJ, Olson PD. Small subunit rDNA and the Platyhelminthes: signals, noise, conflicts and compromise. In: Littlewood DTJ, Bray RA (ed) Interrelationships of the Platyhelminthes. Taylor & Francis, New York. 2001; 262-278
17. Pearson JC, Ow-Yang CK. New species of Haplorchis from Southeast Asia, together with key to the Haplorchis-group of Heterophyid trematode of the region. The Southeast Asian J. Trop. Med. Pub. Health. 1982; 13: 35-60.
18. Pearson JC. A revision of the Subfamily Haplorchinae Looss, 1899 (Trematoda: Heterophyidae). Parasitol. 1964; 54: 601-676.
19. Pinto HA, Melo AL. Melanoides tuberculata (Mollusca: Thiaridae) as an intermediate host of Centrocestus formosanus (Trematoda: Heterophyidae) in Brazil. The Institute of Tropical Medicine. 2010; 52:207-210.
20. Rim H, Sohn WM, Yong TS, Eom KS, Chai JY, Min DY,et al. Fishborne trematode metacercariae detected in freshwater fish from Vientiane Municipality and Savannakhet Province, Lao PDR. Korean J. Parasitol. 2008; 6: 253-260
21. Routtu J, Grunberg D, Izhar R. Selective and universal primers for trematode barcoding in freshwater snails. Parasitol. Res. 2014; 113: 2535-2540.
22. Scholz T, Ditrich O, Giboda M. Differential diagnosis of opisthorchid and heterophyid metacercariae (trematoda) infection of fresh cyprinoid fish from Nam Ngum lake in Laos. The South. Asian J. Trop. Med. Pub. Health. 1991; 22: 171-173.
23. Sorensen RE, Curtis J, Minchella DJ. Intraspecific variation in the rDNA ITS loci of 37 collar-spined echinostomes from North America: implications for sequence based diagnoses and phylogenetics. J. Parasitol. 1998; 84: 992–997.
24. Sukontason K L, Sukontason K, Boonsriwong N, Muangyimpong Y. Intensity of the metacercariae in cyprinid fish in Chaing Mai Province, Northern Thailand. Se Asian J Trop Med. 2001; 32 (suppl 2): 214-217.
25. Van KV, Dalsgaard A, Blair D. Haplorchis pumilio and H. taichui in Vietnam discriminated using ITS-2 DNA sequence data from adult and larvae. Exp. Parasitol. 2009; 123: 146-151.
26. Velasquez CC. Heterophyidiasis, In Steele JH Ed, CRC Handbook Series in Zoonoses, Section C: Parasitic Zoonoses, Vol. III (Trematode Zoonoses). Boca Raton, USA. CRC Press. 1982; pp.99-107.
27. Wongsawad C, Wongsawad P, Chai J. Haplorchis taichui Witenberg, 1930: Development of a HAT-RAPD marker for the detection of minute intestinal fluke infection. Exp. Parasitol. 2009; 123: 158-161.
28. World Health Organization. Food-borne trematode infections in Asia. Report Joint WHO/FAO Workshop, Hanoi, Vietnam. 2002.
29. Yu SH, Mott KE. Epidemiology and morbidity of food-borne intestinal trematode infections. Tropical Dis. Bull. 1994; 91: 125-152.
2.Amaya-Huerta D, Almeyda-Artigas J. Confirmation of Centrocestus formosanus (Nishigori, 1924) Price, 1932 (Trematoda: Heterophyidae) in Mexico. Res. Rev. Parasitol. 1994; 54: 99-103.
3. Anderson GR. Identification and maturation of the metacercaria of Indodidymozoon pearsoni. J. Helminthol. 1999; 73:21–26.
4. Bartoli P, Jousson O, Russell-Pinto F. The life cycle of Monorchis parvus (Digenea: Monorchiidae) demonstrated by developmental and molecular data. J. Parasitol. 2000; 86: 479–489.
5.Belizario VY, Jr de Leon WU, Bersabe MJ. A focus of human infection by Haplorchis taichui (Trematoda: Heterophyidae) in the southern Philippines. J. Parasitol. 2004; 90: 1165-1169.
6. Boonmekam D, Namchote S, Nak-ai W, Glaubrecht M, Krailas D. The Prevalence of Human Intestinal Fluke Infections, Haplorchis taichui, in Thiarid Snails and Cyprinid Fish in Bo Kluea District and Pua District, Nan Province, Thailand. Silpakorn Uni. Science and Technol. J. 2016 ; 10:29-37
7. Bray RA, Gibson D, Jones A. Keys to the Trematoda. London: CAB International and Natural History Museum v. 3. 2008.
8. Chai JY, Murrell KD, Lambery AJ. Fish-borne parasitic zoonoses: status and issues. Int. J. Parasitol. 2005; 35: 1233-1254.
9.Chai JY. Intestinal flukes. In Murrell KD, Fried B eds, Food-borne Parasitic Zoonoses: Fish and Plant-borne Parasites. World Class Parasites, Vol. 11. New York, USA, Springer. 2007 ; 53-115.
10. Cribb TH, Anderson GR, Adlard RD, Bray RA. A DNAbased demonstration of a three host life-cycle for the Bivesiculidae (Platyhelminthes: Digenea). Inter. J. Parasitol. 1998; 28:1791–1795.
11. Dung DT, De NV, Waikagul J. Fishborne zoonotic intestinal trematodes. Emerg. Infect. Dis. j. 2007; 13: 1828–1833.
12.Golchin Manshadi AR, Mirghafari S. Composition and distribution of macrobenthic invertebrates of Shapour River, Fars south-west, Iran. IJBPAS. 2015; 4: 5004-5012.
13. Hillis DM, Dixon, MT. Ribosomal DNA: molecular evolution and phylogenetic inference. Quart. Rev. Biol. 1991; 66:411–453.
14. Jousson O, Bartoli P, Zaninetti L, Pawlowski J. Use of the ITS rDNA for elucidation of some life cycles of mesometridae (Trematoda, Digenea). Inter. J. Parasitol. 1998; 28:1403–1411.
15. Levy MG, Flowers JR, Poore MF, Mullen JE, Khoo L, Pote LM, et al. Morphologic, pathologic, and genetic investigations of Bolbophorus spp. (Diplostomatida, Trematoda) affecting cultured Ictalurus punctatus in the Mississippi Delta. J. Aqu. Animal Health. 2002; 14:235–246.
16. Littlewood DTJ, Olson PD. Small subunit rDNA and the Platyhelminthes: signals, noise, conflicts and compromise. In: Littlewood DTJ, Bray RA (ed) Interrelationships of the Platyhelminthes. Taylor & Francis, New York. 2001; 262-278
17. Pearson JC, Ow-Yang CK. New species of Haplorchis from Southeast Asia, together with key to the Haplorchis-group of Heterophyid trematode of the region. The Southeast Asian J. Trop. Med. Pub. Health. 1982; 13: 35-60.
18. Pearson JC. A revision of the Subfamily Haplorchinae Looss, 1899 (Trematoda: Heterophyidae). Parasitol. 1964; 54: 601-676.
19. Pinto HA, Melo AL. Melanoides tuberculata (Mollusca: Thiaridae) as an intermediate host of Centrocestus formosanus (Trematoda: Heterophyidae) in Brazil. The Institute of Tropical Medicine. 2010; 52:207-210.
20. Rim H, Sohn WM, Yong TS, Eom KS, Chai JY, Min DY,et al. Fishborne trematode metacercariae detected in freshwater fish from Vientiane Municipality and Savannakhet Province, Lao PDR. Korean J. Parasitol. 2008; 6: 253-260
21. Routtu J, Grunberg D, Izhar R. Selective and universal primers for trematode barcoding in freshwater snails. Parasitol. Res. 2014; 113: 2535-2540.
22. Scholz T, Ditrich O, Giboda M. Differential diagnosis of opisthorchid and heterophyid metacercariae (trematoda) infection of fresh cyprinoid fish from Nam Ngum lake in Laos. The South. Asian J. Trop. Med. Pub. Health. 1991; 22: 171-173.
23. Sorensen RE, Curtis J, Minchella DJ. Intraspecific variation in the rDNA ITS loci of 37 collar-spined echinostomes from North America: implications for sequence based diagnoses and phylogenetics. J. Parasitol. 1998; 84: 992–997.
24. Sukontason K L, Sukontason K, Boonsriwong N, Muangyimpong Y. Intensity of the metacercariae in cyprinid fish in Chaing Mai Province, Northern Thailand. Se Asian J Trop Med. 2001; 32 (suppl 2): 214-217.
25. Van KV, Dalsgaard A, Blair D. Haplorchis pumilio and H. taichui in Vietnam discriminated using ITS-2 DNA sequence data from adult and larvae. Exp. Parasitol. 2009; 123: 146-151.
26. Velasquez CC. Heterophyidiasis, In Steele JH Ed, CRC Handbook Series in Zoonoses, Section C: Parasitic Zoonoses, Vol. III (Trematode Zoonoses). Boca Raton, USA. CRC Press. 1982; pp.99-107.
27. Wongsawad C, Wongsawad P, Chai J. Haplorchis taichui Witenberg, 1930: Development of a HAT-RAPD marker for the detection of minute intestinal fluke infection. Exp. Parasitol. 2009; 123: 158-161.
28. World Health Organization. Food-borne trematode infections in Asia. Report Joint WHO/FAO Workshop, Hanoi, Vietnam. 2002.
29. Yu SH, Mott KE. Epidemiology and morbidity of food-borne intestinal trematode infections. Tropical Dis. Bull. 1994; 91: 125-152.
Archivos adicionales
Publicado
2021-03-05
Cómo citar
1.
Golchin Manshadi AR. IDENTIFICACIÓN MORFOLÓGICA Y MOLECULAR DE METACERCARIAE DEL TREMATODO ZOONÓTICO (Haplorchis taitui) EN PECES DE AGUA DULCE EN IRÁN. RVZ [Internet]. 5 de marzo de 2021 [citado 22 de julio de 2024];28:1-9. Disponible en: https://rvz.emnuvens.com.br/rvz/article/view/442
Número
Sección
Artículos Originales
Licencia
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional.
