MORPHOLOGICAL AND MOLECULAR IDENTIFICATION OF ZOONOTIC TREMATODE METACERCARIAE (Haplorchis taitui) IN FRESHWATER FISH IN IRAN
DOI:
https://doi.org/10.35172/rvz.2021.v28.442Keywords:
Trematoda, Heterophyidae, Shapour River, PCRAbstract
The intestinal trematode, Haplorchis taichui (Nishigori, 1924), is an important in public health that causes infection in humans and animals especially in Asia and in parts of Africa and the Americas. Haplorchis taitui metacercariae were found in the gills of Cyprinion macrostomus (Heckel) and Capoeta barroisi persica (Karaman) collected from the Shapour River. Morphological excysted metacercariae were identified as wet mounts under a stereomicroscope. Then, the samples were subjected to molecular analysis. The result showed that 69% of examined fish (n = 30) were diagnosed infected with encysted metacercariae in gills. The mean intensity was 8.3 ± 16.9 parasites per fish. The morphometrical values agree with the findings of other studies with the small differences and polymerase chain reaction product length and nucleotide sequence analysis of 18S ribosomal deoxyribonucleic acid gene showed a similarity of over 99% between the specimens and the Haplorchis taichui (Nishigori, 1924) recorded in GenBank.
References
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25. Van KV, Dalsgaard A, Blair D. Haplorchis pumilio and H. taichui in Vietnam discriminated using ITS-2 DNA sequence data from adult and larvae. Exp. Parasitol. 2009; 123: 146-151.
26. Velasquez CC. Heterophyidiasis, In Steele JH Ed, CRC Handbook Series in Zoonoses, Section C: Parasitic Zoonoses, Vol. III (Trematode Zoonoses). Boca Raton, USA. CRC Press. 1982; pp.99-107.
27. Wongsawad C, Wongsawad P, Chai J. Haplorchis taichui Witenberg, 1930: Development of a HAT-RAPD marker for the detection of minute intestinal fluke infection. Exp. Parasitol. 2009; 123: 158-161.
28. World Health Organization. Food-borne trematode infections in Asia. Report Joint WHO/FAO Workshop, Hanoi, Vietnam. 2002.
29. Yu SH, Mott KE. Epidemiology and morbidity of food-borne intestinal trematode infections. Tropical Dis. Bull. 1994; 91: 125-152.
2.Amaya-Huerta D, Almeyda-Artigas J. Confirmation of Centrocestus formosanus (Nishigori, 1924) Price, 1932 (Trematoda: Heterophyidae) in Mexico. Res. Rev. Parasitol. 1994; 54: 99-103.
3. Anderson GR. Identification and maturation of the metacercaria of Indodidymozoon pearsoni. J. Helminthol. 1999; 73:21–26.
4. Bartoli P, Jousson O, Russell-Pinto F. The life cycle of Monorchis parvus (Digenea: Monorchiidae) demonstrated by developmental and molecular data. J. Parasitol. 2000; 86: 479–489.
5.Belizario VY, Jr de Leon WU, Bersabe MJ. A focus of human infection by Haplorchis taichui (Trematoda: Heterophyidae) in the southern Philippines. J. Parasitol. 2004; 90: 1165-1169.
6. Boonmekam D, Namchote S, Nak-ai W, Glaubrecht M, Krailas D. The Prevalence of Human Intestinal Fluke Infections, Haplorchis taichui, in Thiarid Snails and Cyprinid Fish in Bo Kluea District and Pua District, Nan Province, Thailand. Silpakorn Uni. Science and Technol. J. 2016 ; 10:29-37
7. Bray RA, Gibson D, Jones A. Keys to the Trematoda. London: CAB International and Natural History Museum v. 3. 2008.
8. Chai JY, Murrell KD, Lambery AJ. Fish-borne parasitic zoonoses: status and issues. Int. J. Parasitol. 2005; 35: 1233-1254.
9.Chai JY. Intestinal flukes. In Murrell KD, Fried B eds, Food-borne Parasitic Zoonoses: Fish and Plant-borne Parasites. World Class Parasites, Vol. 11. New York, USA, Springer. 2007 ; 53-115.
10. Cribb TH, Anderson GR, Adlard RD, Bray RA. A DNAbased demonstration of a three host life-cycle for the Bivesiculidae (Platyhelminthes: Digenea). Inter. J. Parasitol. 1998; 28:1791–1795.
11. Dung DT, De NV, Waikagul J. Fishborne zoonotic intestinal trematodes. Emerg. Infect. Dis. j. 2007; 13: 1828–1833.
12.Golchin Manshadi AR, Mirghafari S. Composition and distribution of macrobenthic invertebrates of Shapour River, Fars south-west, Iran. IJBPAS. 2015; 4: 5004-5012.
13. Hillis DM, Dixon, MT. Ribosomal DNA: molecular evolution and phylogenetic inference. Quart. Rev. Biol. 1991; 66:411–453.
14. Jousson O, Bartoli P, Zaninetti L, Pawlowski J. Use of the ITS rDNA for elucidation of some life cycles of mesometridae (Trematoda, Digenea). Inter. J. Parasitol. 1998; 28:1403–1411.
15. Levy MG, Flowers JR, Poore MF, Mullen JE, Khoo L, Pote LM, et al. Morphologic, pathologic, and genetic investigations of Bolbophorus spp. (Diplostomatida, Trematoda) affecting cultured Ictalurus punctatus in the Mississippi Delta. J. Aqu. Animal Health. 2002; 14:235–246.
16. Littlewood DTJ, Olson PD. Small subunit rDNA and the Platyhelminthes: signals, noise, conflicts and compromise. In: Littlewood DTJ, Bray RA (ed) Interrelationships of the Platyhelminthes. Taylor & Francis, New York. 2001; 262-278
17. Pearson JC, Ow-Yang CK. New species of Haplorchis from Southeast Asia, together with key to the Haplorchis-group of Heterophyid trematode of the region. The Southeast Asian J. Trop. Med. Pub. Health. 1982; 13: 35-60.
18. Pearson JC. A revision of the Subfamily Haplorchinae Looss, 1899 (Trematoda: Heterophyidae). Parasitol. 1964; 54: 601-676.
19. Pinto HA, Melo AL. Melanoides tuberculata (Mollusca: Thiaridae) as an intermediate host of Centrocestus formosanus (Trematoda: Heterophyidae) in Brazil. The Institute of Tropical Medicine. 2010; 52:207-210.
20. Rim H, Sohn WM, Yong TS, Eom KS, Chai JY, Min DY,et al. Fishborne trematode metacercariae detected in freshwater fish from Vientiane Municipality and Savannakhet Province, Lao PDR. Korean J. Parasitol. 2008; 6: 253-260
21. Routtu J, Grunberg D, Izhar R. Selective and universal primers for trematode barcoding in freshwater snails. Parasitol. Res. 2014; 113: 2535-2540.
22. Scholz T, Ditrich O, Giboda M. Differential diagnosis of opisthorchid and heterophyid metacercariae (trematoda) infection of fresh cyprinoid fish from Nam Ngum lake in Laos. The South. Asian J. Trop. Med. Pub. Health. 1991; 22: 171-173.
23. Sorensen RE, Curtis J, Minchella DJ. Intraspecific variation in the rDNA ITS loci of 37 collar-spined echinostomes from North America: implications for sequence based diagnoses and phylogenetics. J. Parasitol. 1998; 84: 992–997.
24. Sukontason K L, Sukontason K, Boonsriwong N, Muangyimpong Y. Intensity of the metacercariae in cyprinid fish in Chaing Mai Province, Northern Thailand. Se Asian J Trop Med. 2001; 32 (suppl 2): 214-217.
25. Van KV, Dalsgaard A, Blair D. Haplorchis pumilio and H. taichui in Vietnam discriminated using ITS-2 DNA sequence data from adult and larvae. Exp. Parasitol. 2009; 123: 146-151.
26. Velasquez CC. Heterophyidiasis, In Steele JH Ed, CRC Handbook Series in Zoonoses, Section C: Parasitic Zoonoses, Vol. III (Trematode Zoonoses). Boca Raton, USA. CRC Press. 1982; pp.99-107.
27. Wongsawad C, Wongsawad P, Chai J. Haplorchis taichui Witenberg, 1930: Development of a HAT-RAPD marker for the detection of minute intestinal fluke infection. Exp. Parasitol. 2009; 123: 158-161.
28. World Health Organization. Food-borne trematode infections in Asia. Report Joint WHO/FAO Workshop, Hanoi, Vietnam. 2002.
29. Yu SH, Mott KE. Epidemiology and morbidity of food-borne intestinal trematode infections. Tropical Dis. Bull. 1994; 91: 125-152.
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2021-03-05
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Golchin Manshadi AR. MORPHOLOGICAL AND MOLECULAR IDENTIFICATION OF ZOONOTIC TREMATODE METACERCARIAE (Haplorchis taitui) IN FRESHWATER FISH IN IRAN. RVZ [Internet]. 2021 Mar. 5 [cited 2024 Jul. 22];28:1-9. Available from: https://rvz.emnuvens.com.br/rvz/article/view/442
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