EFICIÊNCIA DE TRÊS DILUIDORES INESPECIFICOS NA REFRIGERAÇÃO DO SEMEN DE COELHO (Oryctolagus cuniculus f. domesticus)

Autores

  • NATAN DIAS DE OLIVEIRA Instituto Federal do Norte de Minas Gerais - Campus Salinas/Graduando em Medicina Veterinária https://orcid.org/0000-0001-5446-6744
  • Pablo Arruda Caires Instituto Federal do Norte de Minas Gerais - Campus Salinas/Graduando em Medicina Veterinária
  • Fernanda Cristine Figueiredo Fernandes Instituto Federal do Norte de Minas Gerais - Campus Salinas/Mestranda https://orcid.org/0000-0002-9361-5885
  • Júlio César Oliveira Dias Instituto Federal do Norte de Minas Gerais - Campus Salinas/Docente https://orcid.org/0000-0002-9356-4264
  • Ana Carolina Leite Albeny Instituto Federal do Norte de Minas Gerais - Campus Salinas/Docente https://orcid.org/0000-0003-1374-7106

DOI:

https://doi.org/10.35172/rvz.2023.v30.1368

Palavras-chave:

crioprotetor, cunicultura, diluidor, espermatozoide, inseminação artificial

Resumo

Objetivou-se com este trabalho avaliar a viabilidade seminal de coelhos após refrigeração a 5°C utilizando três diluentes comerciais, indicados para refrigeração de sêmen de outras espécies monogástricas. Foram realizadas dez colheitas de sêmen de cada reprodutor. Os ejaculados de cada coelho eram misturados, obtendo-se um pool de sêmen. Em seguida, o pool heterospérmico foi dividido em três tratamentos, de acordo com o diluente utilizado: BotuDog® (cães); BotuSemen® (equinos); e Beltsville Thawing Solution – BTS ® (suínos). As amostras foram avaliadas quanto a parâmetros macroscópicos, microscópicos e funcionais em quatro momentos: sêmen fresco (0 h) e refrigerado (16, 24 e 48 h).  Os valores médios de motilidade, vigor e defeitos totais do sêmen fresco foram de 86,0%, 3,05 e 11,5%, respectivamente. Após o início do resfriamento, observou-se comportamento linear decrescente para a motilidade e vigor espermático do sêmen em função do tempo, diferindo (P < 0,05) entre os tratamentos. Destaca-se que o tratamento diluído em BTS ®  apresentou a redução na manutenção dos parâmetros avaliados de forma mais pronunciada (P < 0,05) ao longo do tempo, apresentando os menores valores para motilidade e vigor espermático dentro de cada tempo avaliado: 16h [20,6 ± 7,28 e 0,75 (0,375 - 1,5)], 24h [12,0 ± 6,80 e 0 (0 - 1,0)] e 48h [3,0 ± 2,13 e 0 (0 - 0,125)]. Após 48 h de resfriamento, o BotuDog® foi o que apresentou maior valor para motilidade espermática (71,0 ± 2,08) e, juntamente, com o BotuSemen® apresentaram maior valor para vigor espermático (2.5). De acordo com os dados obtidos os diluentes BotuDog® e BotuSemen® mostraram-se eficientes em manter parâmetros espermáticos adequados, apresentando boa viabilidade espermática em até 48h pós refrigeração a 5°C.  Obteve-se através deste trabalho, resultados inéditos quanto a parâmetros espermáticos do sêmen de coelho refrigerado que podem ser utilizados como valores de referência para o manejo reprodutivo e processamento do semen desses animais, visto a inexistênca dessas  recomendações técnicas.

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2023-08-07

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DIAS DE OLIVEIRA N, Arruda Caires P, Cristine Figueiredo Fernandes F, Oliveira Dias JC, Leite Albeny AC. EFICIÊNCIA DE TRÊS DILUIDORES INESPECIFICOS NA REFRIGERAÇÃO DO SEMEN DE COELHO (Oryctolagus cuniculus f. domesticus). RVZ [Internet]. 7º de agosto de 2023 [citado 5º de dezembro de 2024];30:1-14. Disponível em: https://rvz.emnuvens.com.br/rvz/article/view/1368

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