INCORPORAÇÃO DE Lacticaseibacillus paracasei PROBIÓTICO EM SHAMPOO PARA PEQUENOS ANIMAIS

Autores

DOI:

https://doi.org/10.35172/rvz.2025.v32.1686

Palavras-chave:

bioatividade, Lacticaseibacillus, pets, shampoo funcional.

Resumo

Probióticos são microrganismos vivos que apresentam potencial para prevenção e tratamento de doenças dermatológicas. Em medicina veterinária, sua administração de forma tópica ainda precisa ser explorada. O objetivo deste trabalho foi avaliar a incorporação do isolado probiótico Lacticaseibacillus paracasei M1A3 em uma matriz shampoo para tratamento em pequenos animais. Para tal, analisou-se a atividade antimicrobiana frente a microrganismos de interesse veterinário, formação de biofilme, suscetibilidade a antimicrobianos e produção de exopolissacarídeos. A manutenção da viabilidade de L. paracasei M1A3 foi avaliada por 28 dias através do grupo controle (G1) e grupo matriz shampoo (G2). A bactéria probiótica apresentou atividade antimicrobiana contra Candida albicans ATCC 90022, Corynebacterium fimi NCTC 7547, Corynebacterium sp. SA1-2, Listeria monocytogenes 17D78/03 e Listeria monocytogenes ATCC 7644, com halos entre 11,7 e 23 mm. Também apresentou forte formação de biofilme, suscetibilidade a quase todos antimicrobianos testados e produção de exopolissacarídeos. As contagens de G1 e G2 foram de, respectivamente, 2,45 x 107 UFC/mL e 2,02 x 107 UFC/mL no momento da incorporação, 1,35 x 108 UFC/mL e 1,75 x 106 UFC/mL aos 7 dias, 4,06 x 108 UFC/mL e 1,30 x 105 UFC/mL aos 14 dias, 1,69 x 107 UFC/mL e 5,34 x 104 UFC/mL aos 21 dias e 8,60 x 105 UFC/mL e 6,97 x 103 UFC/mL aos 28 dias, respectivamente. Conclui-se que o isolado apresentou potencial para aplicação em um shampoo probiótico terapêutico para pequenos animais, com estabilidade por aproximadamente 21 dias no grupo controle. Contudo, outros estudos devem ser ampliados para prospectar outras formas de incorporação, como, por exemplo, a microencapsulação do isolado probiótico.

Referências

Food and Agricultural Organization of the United Nations and World Health Organization. Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food. London: FAO/WHO; 2002.

Grześkowiak Ł, Endo A, Beasley S, Salminen S. Microbiota and probiotics in canine and feline welfare. Anaerobe. 2015 Aug;34:14–23. doi: 10.1016/j.anaerobe.2015.04.002

Habeebuddin M, Karnati RK, Shiroorkar PN, Nagaraja S, Asdaq SMB, Khalid Anwer Md, et al. Topical Probiotics: More Than a Skin Deep. Pharmaceutics. 2022 Mar 3;14(3):557. doi: 10.3390/pharmaceutics14030557

Dou J, Feng N, Guo F, Chen Z, Liu J, Wang T, et al. Applications of Probiotic Constituents in Cosmetics. Molecules. 2023 Sep 22;28(19):6765–5. doi: 10.3390/molecules28196765

Drechsler Y, Dong C, Clark D, Kaur G. Canine Atopic Dermatitis: Prevalence, Impact, and Management Strategies. Veterinary Medicine: Research and Reports. 2024 Feb 1;Volume 15:15–29. doi: 10.2147/VMRR.S412570

Gedon NKY, Mueller RS. Atopic dermatitis in cats and dogs: a difficult disease for animals and owners. Clin Transl Allergy. 2018 Oct 5;8:41. doi: 10.1186/s13601-018-0228-5

Gelmetti C, Rigoni C, Cantù A, Agolzer A, Agrusa A, Brena M, et al. Topical prebiotics/postbiotics and PRURISCORE validation in atopic dermatitis. International study of 396 patients. 2022 Oct 17;34(1). doi: 10.1080/09546634.2022.2131703

Kawano K, Iyori K, Kondo N, Yamakawa S, Fujii T, Funasaka K, et al. Clinical effects of combined Lactobacillus paracasei and kestose on canine atopic dermatitis. Polish Journal of Veterinary Sciences. 2023 Mar 1;26(1):131–6. doi: 10.24425/pjvs.2023.145014

Ohshima-Terada Y, Higuchi Y, Kumagai T, Hagihara A, Nagata M. Complementary effect of oral administration of Lactobacillus paracasei K71 on canine atopic dermatitis. Veterinary Dermatology. 2015 Jun 30;26(5):350-e75. doi: 10.1111/vde.12224

Schilling J, Mueller RS. Double-blinded, placebo-controlled study to evaluate an antipruritic shampoo for dogs with allergic pruritus. Veterinary Record. 2012 Jul;171(4):97–7. doi: 10.1136/vr.100635

Purutoğlu K, İspirli H, Yüzer MO, Serencam H, Dertli E. Diversity and functional characteristics of lactic acid bacteria from traditional kefir grains. International Journal of Dairy Technology. 2019 Aug 4;73(1):57–66. doi: 10.1111/1471-0307.12633

Mariana Muller Giacon, Franciele Maboni Siqueira, Amanda. Microbial Contamination and Antimicrobial Resistance Profiles Indicate Potential Risks of Infection at the Veterinary Medical Teaching Hospital - UFRGS, Porto Alegre, Brazil. Acta Scientiae Veterinariae. 2021 Jan 1;49. doi: 10.22456/1679-9216.108992

Stepanovic S, Cirkovic I, Ranin L, Svabic-Vlahovic M. Biofilm formation by Salmonella spp. and Listeria monocytogenes on plastic surface. Letters in Applied Microbiology. 2004 May;38(5):428–32. doi: 10.1111/j.1472-765X.2004.01513.x

Schmidt K, Estes C, McLaren A, Spangehl MJ. Chlorhexidine Antiseptic Irrigation Eradicates Staphylococcus epidermidis From Biofilm: An In Vitro Study. Clinical Orthopaedics & Related Research. 2018 Feb 13;476(3):648–53. doi: 10.1007/s11999.0000000000000052

Masebe RD, Thantsha MS. Anti-Biofilm Activity of Cell Free Supernatants of Selected Lactic Acid Bacteria against Listeria monocytogenes Isolated from Avocado and Cucumber Fruits, and from an Avocado Processing Plant. Foods. 2022 Sep 16;11(18):2872. doi: 10.3390/foods11182872

Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology. 1966 Apr 1;45(4):493–6. doi: 10.1093/ajcp/45.4_ts.493

Charteris WP, Kelly PM, Morelli L, Collins JK. Antibiotic Susceptibility of Potentially Probiotic Lactobacillus Species. Journal of Food Protection. 1998 Dec;61(12):1636–43. doi: 10.4315/0362-028x-61.12.1636

Freeman DJ, Falkiner FR, Keane CT. New method for detecting slime production by coagulase negative staphylococci. Journal of Clinical Pathology. 1989 Aug 1;42(8):872–4. doi: 10.1136/jcp.42.8.872

Miles AA, Misra SS, Irwin JO. The estimation of the bactericidal power of the blood. Epidemiology and Infection. 1938 Nov;38(6):732–49. doi: 10.1017/s002217240001158x

Reuben RC, Roy PC, Sarkar SL, Rubayet Ul Alam ASM, Jahid IK. Characterization and evaluation of lactic acid bacteria from indigenous raw milk for potential probiotic properties. Journal of Dairy Science. 2019 Nov;103(2). doi: 10.3168/jds.2019-17092

Falfán-Cortés RN, Mora-Peñaflor N, Gómez-Aldapa CA, Rangel-Vargas E, Acevedo-Sandoval OA, Franco-Fernández MJ, et al. Characterization and Evaluation of the Probiotic Potential In Vitro and In Situ of Lacticaseibacillus paracasei Isolated from Tenate Cheese. Journal of Food Protection. 2022 Jan 1;85(1):112–21. doi: 10.4315/JFP-21-021

Shahverdi S, Barzegari AA, Vaseghi Bakhshayesh R, Nami Y. In-vitro and in-vivo antibacterial activity of potential probiotic Lactobacillus paracasei against Staphylococcus aureus and Escherichia coli. Heliyon. 2023 Apr 1;9(4):e14641. doi: 10.1016/j.heliyon.2023.e14641

Ribeiro F de C, Junqueira JC, dos Santos JD, de Barros PP, Rossoni RD, Shukla S, et al. Development of Probiotic Formulations for Oral Candidiasis Prevention: Gellan Gum as a Carrier To Deliver Lactobacillus paracasei 28.4. Antimicrobial Agents and Chemotherapy. 2020 Apr 6;64(6). doi: 10.1128/AAC.02323-19

Di Domenico EG, Cavallo I, Capitanio B, Ascenzioni F, Pimpinelli F, Morrone A, et al. Staphylococcus aureus and the Cutaneous Microbiota Biofilms in the Pathogenesis of Atopic Dermatitis. Microorganisms. 2019 Aug 29;7(9):301. doi: 10.3390/microorganisms7090301

Baliyan N, Dindhoria K, Kumar A, Thakur A, Kumar R. Comprehensive Substrate-Based Exploration of Probiotics From Undistilled Traditional Fermented Alcoholic Beverage “Lugri”. Frontiers in Microbiology. 2021 Mar 12;12. doi: 10.3389/fmicb.2021.626964

Gueimonde M, Sánchez B, G de Los Reyes-Gavilán C, Margolles A. Antibiotic resistance in probiotic bacteria. Frontiers in microbiology. 2013 Jul 18;4:202. doi: 10.3389/fmicb.2013.00202

Anisimova EA, Yarullina DR. Antibiotic Resistance of LACTOBACILLUS Strains. Current Microbiology. 2019 Sep 25;76(12):1407–16. doi: 10.1007/s00284-019-01769-7

Kıray E, Raheel NM. A New Approach to Exopolysaccharides of Post Probiotic Lactobacillus paracasei L1 Strain: Anti-quarum Sensing Activity. Balkan medical journal/Balkan Medical Journal. 2023 Sep 13;40(5):351–7. doi: 10.4274/balkanmedj.galenos.2023.2023-1-96

M’hamed AC, Ncib K, Merghni A, Migaou M, Lazreg H, Snoussi M, et al. Characterization of Probiotic Properties of Lacticaseibacillus paracasei L2 Isolated from a Traditional Fermented Food “Lben.” Life. 2022 Dec 21;13(1):21. doi: 10.3390/life13010021

Arshad T, Mundrathi V, Perez VE, Nunez JM, Cho H. Topical Probiotic Hydrogels for Burn Wound Healing. Gels. 2024 Aug 23;10(9):545. doi: 10.3390/gels10090545

Tao S, Zhang S, Wei K, Maniura‐Weber K, Li Z, Ren Q. An Injectable Living Hydrogel with Embedded Probiotics as a Novel Strategy for Combating Multifaceted Pathogen Wound Infections. Advanced Healthcare Materials. 2024 Oct;13(27):e2400921. doi: 10.1002/adhm.202400921

Mei L, Zhang D, Shao H, Hao Y, Zhang T, Zheng W, et al. Injectable and Self-Healing Probiotics-Loaded Hydrogel for Promoting Superbacteria-Infected Wound Healing. ACS Applied Materials & Interfaces. 2022 Apr 26;14(18):20538–50. doi: 10.1021/acsami.1c23713

Xu H, Li Y, Song J, Zhou L, Wu K, Lu X, et al. Highly active probiotic hydrogels matrixed on bacterial EPS accelerate wound healing via maintaining stable skin microbiota and reducing inflammation. Bioactive Materials. 2024 May 1;35:31–44. doi: 10.1016/j.bioactmat.2024.01.011

Downloads

Publicado

2025-04-10

Como Citar

1.
Agustini E, Souza da Motta A. INCORPORAÇÃO DE Lacticaseibacillus paracasei PROBIÓTICO EM SHAMPOO PARA PEQUENOS ANIMAIS. RVZ [Internet]. 10º de abril de 2025 [citado 17º de abril de 2025];32:1-9. Disponível em: https://rvz.emnuvens.com.br/rvz/article/view/1686

Edição

Seção

Artigos Originais