IDENTIFICAÇÃO MORFOLÓGICA E MOLECULAR DE METACERCÁRIOS DE TREMATODOS ZOONÓTICOS (Haplorchis taichui) EM PEIXES DE ÁGUA DOCE NO IRÃ

Palavras-chave: Trematoda, Heterophyidae, Shapour River, PCR

Resumo

O trematódeo intestinal, Haplorchis taichui (Nishigori, 1924), é importante na saúde pública que causa infecção em humanos e animais, especialmente na Ásia e em partes da África e das Américas. Haplorchis taitui metacercariae foram encontradas nas brânquias de Cyprinion macrostomus (Heckel) e Capoeta barroisi persica (Karaman) coletadas no rio Shapour. Metacercárias morfológicas excisadas foram identificadas como montagens úmidas sob um estereomicroscópio. Em seguida, as amostras foram submetidas à análise molecular. O resultado mostrou que 69% dos peixes examinados (n = 30) foram diagnosticados infectados com metacercárias encistadas em brânquias. A intensidade média foi de 8,3 ± 16,9 parasitas por peixe. Os valores morfométricos concordam com os achados de outros estudos com as pequenas diferenças e o comprimento do produto da reação em cadeia da polimerase e a análise da sequência nucleotídica do gene do ácido desoxirribonucléico ribossômico 18S mostraram uma similaridade de mais de 99% entre as amostras e o Haplorchis taichui (Nishigori, 1924) registrado no GenBank.

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Publicado
05-03-2021
Como Citar
1.
Golchin Manshadi A. IDENTIFICAÇÃO MORFOLÓGICA E MOLECULAR DE METACERCÁRIOS DE TREMATODOS ZOONÓTICOS (Haplorchis taichui) EM PEIXES DE ÁGUA DOCE NO IRÃ. RVZ [Internet]. 5º de março de 2021 [citado 13º de maio de 2021];28:1-. Disponível em: https://rvz.emnuvens.com.br/rvz/article/view/442
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