DIAGNÓSTICO DE LEPTOSPIRA SPP. EM BOVINOS ABATIDOS NO BRASIL

Autores

  • Marcelo Augusto Orsi Dutra Department of Animal Production and Preventive Veterinary Medicine. São Paulo State University – UNESP
  • Amanda Bezerra Bertolini Department of Animal Production and Preventive Veterinary Medicine. São Paulo State University – UNESP https://orcid.org/0000-0002-1575-6770
  • Suzane Manzini Department of Infectology, Dermatology, Diagnostic Imaging and Radiotherapy. Botucatu Medical School. São Paulo State University – UNESP https://orcid.org/0000-0001-8052-5736
  • Livia Maísa Guiraldi Department of Infectology, Dermatology, Diagnostic Imaging and Radiotherapy. Botucatu Medical School. São Paulo State University – UNESP
  • Wesley José dos Santos Department of Infectology, Dermatology, Diagnostic Imaging and Radiotherapy. Botucatu Medical School. São Paulo State University – UNESP
  • Isabella Neves Aires Department of Biology. Faculty of Science. São Paulo State University – UNESP https://orcid.org/0000-0001-5285-3150
  • Nassarah Jabur Lot Rodrigues Department of Infectology, Dermatology, Diagnostic Imaging and Radiotherapy. Botucatu Medical School. São Paulo State University – UNESP https://orcid.org/0000-0003-4970-2903
  • Thainá Valente Bertozzo Department of Infectology, Dermatology, Diagnostic Imaging and Radiotherapy. Botucatu Medical School. São Paulo State University – UNESP https://orcid.org/0000-0002-7971-2021
  • Marcela Alexandrino Faculdade de Medicina de Botucatu https://orcid.org/0000-0001-8968-7694
  • Andresa Xavier Frade Gomes Xavier Frade Gomes Department of Animal Production and Preventive Veterinary Medicine. São Paulo State University – UNESP https://orcid.org/0000-0001-8228-2301
  • Jackieline Sampaio Steinle Department of Animal Production and Preventive Veterinary Medicine. São Paulo State University – UNESP https://orcid.org/0000-0002-7366-2449
  • Maria Eduarda Cavalheiro Department of Infectology, Dermatology, Diagnostic Imaging and Radiotherapy. Botucatu Medical School. São Paulo State University – UNESP https://orcid.org/0000-0003-1306-812X
  • Ana Carolina Yamakawa Department of Animal Production and Preventive Veterinary Medicine. São Paulo State University – UNESP
  • Ana Paula Flaminio Department of Animal Production and Preventive Veterinary Medicine. São Paulo State University – UNESP https://orcid.org/0000-0002-7782-2163
  • Hélio Langoni Department of Animal Production and Preventive Veterinary Medicine. São Paulo State University – UNESP https://orcid.org/0000-0001-5127-0762
  • Antonio Carlos Paes Department of Animal Production and Preventive Veterinary Medicine. São Paulo State University – UNESP
  • Maria Izabel Merino de Medeiros São Paulo’s Agency for Agribusiness Technology (APTA/SAA) https://orcid.org/0000-0001-7902-1872
  • Vera Claudia Lorenzetti Magalhaes Curci São Paulo’s Agency for Agribusiness Technology (APTA/SAA), Biological Institute, Araçatuba https://orcid.org/0000-0003-3393-0493
  • Simone Baldini Lucheis São Paulo’s Agency for Agribusiness Technology (APTA/SAA) https://orcid.org/0000-0002-4842-5281

DOI:

https://doi.org/10.35172/rvz.2022.v29.901

Palavras-chave:

Bovinos, Diagnóstico, Leptospirose, Zoonose

Resumo

A leptospirose é considerada uma zoonose bacteriana de importância para a saúde pública. É comum em áreas tropicais, especialmente em países em desenvolvimento com escassos recursos de saúde e saneamento. Este estudo avaliou a presença de Leptospira spp. em bovinos abatidos em frigorífico da região Centro-Oeste de São Paulo, Brasil, e identificou animais positivos tanto por sorologia quanto em análise molecular. Amostras biológicas de sangue, fígado e rins de 150 bovinos foram investigadas pela técnica de Teste de Aglutinação Microscópica (MAT) e Reação em Cadeia da Polimerase convencional (cPCR). Os resultados sorológicos mostraram que dos 150 animais, 71 (47,3%) foram reativos. Os resultados moleculares mostraram a presença de Leptospira spp. nos rins de 21 animais (14%), no fígado de 5 animais (3,3%), no fígado e rins em 2 animais (1,3%) e no sangue em 1 animal (0,7%). Esses resultados indicam um alerta sobre a saúde dos bovinos de corte devido à possibilidade desses animais serem fonte de infecção e a importância da característica ocupacional desta doença. Verificou-se também a importância de complementar as técnicas sorológicas e moleculares.

Referências

Binns C, Wah YL. Leptospirosis: a public health story encircling the globe. Asia Pac J Public Health. 2016;28(4):288-9. DOI: https://doi.org/10.1177/1010539516648021

Guernier V, Goarant C, Benschop J, Lau CL. A systematic review of human and animal leptospirosis in the Pacific Islands reveals pathogen and reservoir diversity. PLoS Negl Trop Dis. 2018;12(5):e0006503. doi: https://doi.org/10.1371/journal.pntd.0006503. DOI: https://doi.org/10.1371/journal.pntd.0006503

Campos AP, Miranda DFH, Rodrigues HWS, Lustosa MSC, Martins GHC, Mineiro ALBB, et al. Seroprevalence and risk factors for leptospirosis in cattle, sheep, and goats at consorted rearing from the State of Piauí, northeastern Brazil. Trop Anim Health Prod. 2017;49(5):899-907. doi: 10.1007/s11250-017-1255-2. DOI: https://doi.org/10.1007/s11250-017-1255-2

Balassiano IT, Vital-Brazil JM, Ramos TMV, Timm LN, Pereira MM. Molecular and serological characterization of Leptospira kirschneri serogroup Pomona isolated from a human case in a Brazilian rural área. Rev Soc Bras Med Trop. 2017;50(3):396-8. doi: https://doi.org/10.1590/0037-8682-0445-2016. DOI: https://doi.org/10.1590/0037-8682-0445-2016

Fávero JF, Araújo HL, Lilenbaum W, Machado G, Tonin AA, Baldissera MD, et al. Bovine leptospirosis: prevalence, associated risk factors for infection and their cause-effect relation. Microb Pathog. 2017;107:149-54. doi: https://doi.org/10.1016/j.micpath.2017.03.032. DOI: https://doi.org/10.1016/j.micpath.2017.03.032

Mérien F, Amouriaux P, Perolat P, Baranton G, Saint Girons I. Polymerase chain reaction for detection of Leptospira spp in clinical samples. J Clin Microbiol. 1992;30(9):2219-24. doi: https://doi.org/10.1128/jcm.30.9.2219-2224.1992. DOI: https://doi.org/10.1128/jcm.30.9.2219-2224.1992

Manzini SM, Santos WJ, Guiraldi LV, Aires IN, Medeiros MIM, Pereira VBR, et al. First isolation of Leptospira kirschneri serovar Canicola and Leptospira interrogans serovar Pyrogenes in urine samples from slaughtered cattle in Midwest region of São Paulo state, Brazil. Arch Vet Sci. 2021;26(1):51-62. DOI: https://doi.org/10.5380/avs.v26i1.77692

Rajala EL, Nosirjon Sattorov N, Boqvist S, Magnusson U. Bovine leptospirosis in urban and peri-urban dairy farming in low-income countries: a “One Health” issue? Acta Vet Scand. 2017;59(1):83. doi: 10.1186/s13028-017-0352-6. DOI: https://doi.org/10.1186/s13028-017-0352-6

Pinna MH, Martins G, Loureiro AP, Lilenbaum W. Detection of bovine carriers of Leptospira by serological, bacteriological, and molecular tools. Trop Anim Health Prod. 2018, 50(4):883-8. doi: 10.1007/s11250-018-1512-z. DOI: https://doi.org/10.1007/s11250-018-1512-z

Langoni H, Kuribara IY, Correa APFL, Ullmann LS, Sanchez GP, Lucheis SB. Anti-leptospirosis agglutinins in Brazilian capybaras (Hydrochoerus hydrochaeris). J Venom Anim Toxins Incl Trop Dis. 2016;27(22):4. doi: https://doi.org/10.1186/s40409-016-0059-6. DOI: https://doi.org/10.1186/s40409-016-0059-6

Vieira AS, Narduche L, Martins G, Schabib-Peres IA, Zimmermann NP, Juliano RS, et al. Detection of wild animals as carriers of Leptospira by PCR in the Pantanal biome, Brazil. Acta Trop. 2016;163(87):9. doi: https://doi.org/10.1016/j.actatropica.2016.08.001. DOI: https://doi.org/10.1016/j.actatropica.2016.08.001

Dreyfus A, Wilson P, Collins-Emerson J, Benschop J, Moore S, Heuer C. Risk factors for new infection with Leptospira in meat workers in New Zealand. Occup Environ Med. 2015;72(3): 219-25. doi: http://dx.doi.org/10.1136/oemed-2014-102457. DOI: https://doi.org/10.1136/oemed-2014-102457

Maze MJ, Cash-Goldwasser S, Rubach MP, Biggs HM, Galloway RL, Sharples KJ, et al. Risk factors for human acute leptospirosis in northern Tanzania. PLoS Negl Trop Dis. 2018;12(6):e0006372. doi: https://doi.org/10.1371/journal.pntd.0006372. DOI: https://doi.org/10.1371/journal.pntd.0006372

Loureiro AP, Pestana C, Medeiros MA, Lilenbaum W. High frequency of leptospiral vaginal carriers among slaughtered cows. Anim Reprod Sci. 2017;178(1):50-4. doi: https://doi.org/10.1016/j.anireprosci.2017.01.008. DOI: https://doi.org/10.1016/j.anireprosci.2017.01.008

Pinto PS, Loureiro AP, Penna B, Lilenbaum W. Usage of Leptospira spp. local strains as antigenes increases the sensitivity of the serodiagnosis of bovine leptospirosis. Acta Trop. 2015;149(1):163-7. doi: https://doi.org/10.1016/j.actatropica.2015.05.008. DOI: https://doi.org/10.1016/j.actatropica.2015.05.008

Li S, Subbiah J, Dvorak B. Environmental and occupational impacts from U.S. beef slaughtering are of same magnitude of beef foodborne illnesses on human health. Environ Int. 2019;129(1):507-51. doi: https://doi.org/10.1016/j.envint.2019.05.051. DOI: https://doi.org/10.1016/j.envint.2019.05.051

Feltes MMC, Arisseto-Bragotto AP, Block JM. Food quality, food-borne diseases, and food safety in the Brazilian food industry. Food Qual Saf. 2017;1:13-27. doi: https://doi.org/10.1093/fqsafe/fyx003 DOI: https://doi.org/10.1093/fqs/fyx003

Gazzonis AL, Marino AMF, Garippa G, Rossi L, Mignone W, Dini V, et al. Toxoplasma gondii seroprevalence in beef cattle raised in Italy: a multicenter study. Parasitol Res. 2020;119(11):3893-8. doi: https://doi.org/10.1007/s00436-020-06878-y. DOI: https://doi.org/10.1007/s00436-020-06878-y

Gutema FD, Shiberu T, Agga GE, Abdi RD, Hiko A, Tufa TB, et al. Bovine cysticercosis and human taeniasis in a rural community in Ethiopia. Zoonoses Public Health. 2020;67(5):525-33 doi: https://doi.org/10.1111/zph.12716. DOI: https://doi.org/10.1111/zph.12716

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Publicado

2022-10-31

Como Citar

1.
Orsi Dutra MA, Bezerra Bertolini A, Manzini S, Guiraldi LM, dos Santos WJ, Neves Aires I, Rodrigues NJL, Valente Bertozzo T, Alexandrino M, Xavier Frade Gomes AXFG, Sampaio Steinle J, Cavalheiro ME, Yamakawa AC, Flaminio AP, Langoni H, Paes AC, Merino de Medeiros MI, Lorenzetti Magalhaes Curci VC, Baldini Lucheis S. DIAGNÓSTICO DE LEPTOSPIRA SPP. EM BOVINOS ABATIDOS NO BRASIL. RVZ [Internet]. 31º de outubro de 2022 [citado 21º de dezembro de 2024];29:1-8. Disponível em: https://rvz.emnuvens.com.br/rvz/article/view/901

Edição

v. 29 (2022)

Seção

Artigos Originais

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Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional.

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