EVALUATION OF CANINE TOXOPLASMOSIS SEROPREVALENCE IN ANIMALS FROM GREATER VITÓRIA-ES

Authors

  • Ana Caroline Moschen Batista Universidade Vila Velha, UVV, Vila Velha, Espírito Santo, Brasil
  • Gismelli Cristiane Angeluci Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina Veterinária e Zootecnia, UNESP-FMVZ, Botucatu https://orcid.org/0000-0001-6540-6050
  • Nassarah Jabur Lot Rodrigues Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina, UNESP-FMB, Botucatu https://orcid.org/0000-0003-4970-2903
  • Gabrielle dos Santos Rocha Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina Veterinária e Zootecnia, UNESP-FMVZ, Botucatu https://orcid.org/0000-0001-8052-7109
  • Suellen Gonçalves Lima Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina Veterinária e Zootecnia, UNESP-FMVZ, Botucatu
  • Fabio Ribeiro Braga Universidade Vila Velha, UVV, Vila Velha, ES https://orcid.org/0000-0003-2942-5785
  • Emy Hiura Universidade Vila Velha, UVV, Vila Velha, ES https://orcid.org/0000-0002-6502-3292
  • Luiz Daniel de Barros Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina Veterinária e Zootecnia, UNESP-FMVZ, Botucatu https://orcid.org/0000-0002-8560-3948
  • Aristeu Vieira da Silva Universidade Estadual de Feira de Santana, Feira de Santana, Bahia https://orcid.org/0000-0003-3842-2279
  • Benedito Donizete Menozzi Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina, UNESP-FMB, Botucatu
  • Helio Langoni Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Medicina Veterinária e Zootecnia, UNESP-FMVZ, Botucatu https://orcid.org/0000-0001-5127-0762

DOI:

https://doi.org/10.35172/rvz.2023.v30.1569

Keywords:

dogs, sentinel, serology, zoonosis

Abstract

Toxoplasmosis is a zoonosis of worldwide distribution, and dogs, despite intermediate hosts, participate in the epidemiological chain of transmission, both to other animals and humans. As an epidemiological surveillance activity, a serological research was carried out for antibodies of the IgG class anti-Toxoplasma gondii in serum samples obtained from 150 dogs, from January to March 2022, belonging to the serum bank of the Veterinary Clinical Laboratory, of the Faculty of Veterinary Medicine of  UVV, Vila Velha-ES. The indirect fluorescent antibody test (IFAT-IgG) was used, evaluating the results in relation to the titers obtained, age, and gender. Sixteen (10.66%) of the samples were positive, with variable titers between 16 and 256, 15 (93.75%) with title 16, 11 (68.75%) 64, and three (18.75%) 256. Regarding gender, of the 16 positive animals, nine (56.25%) were females, and seven (43.75%) were males. Related to age, positive animals ranged from three to 15 years, and animals with higher titles were between 10 and 12 years old. It is concluded that despite the low seroprevalence, T. gondii dispersion occurs in the studied region and that surveillance measures should be established to control infection and disease in the canine and human populations.

References

Figueiredo HR, Favero S, Amendoeira MRR, Cardozo C. Seroepidemiological survey of toxoplasmosis and evaluation of the conditioning factors for its transmission in undergraduate students from Campo Grande, Mato Grosso do Sul State, Brazil. Scientia Medica. 2010;20(1):71-75.

Silva AV, Pezerico SB, Lima VY, Moretti L d’Arc, Pinheiro JP, Tanaka EM, et al. Genotyping of Toxoplasma gondii strains isolated from dogs with neurological signs. Vet. Parasitol. 2005;127(1):2327. doi:10.1016/j.vetpar.2004.08.020. DOI: https://doi.org/10.1016/j.vetpar.2004.08.020

Silva RC, Langoni H, Su C, Silva AV. Genotypic characterization of Toxoplasma gondii in sheep from Brazilian slaughterhouses: New atypical genotypes and the clonal type II strain identified. Vet. Parasitol. 2011;175(1-2):173177. doi: 10.1016/j.vetpar.2010.09.021. DOI: https://doi.org/10.1016/j.vetpar.2010.09.021

Frenkel JK, Parker BB. An apparent role of dogs in the transmission of Toxoplasma gondii the probable importance of xenosmophilia. In: Annals New York Academy of Sciences. 1996; 402-407. DOI: https://doi.org/10.1111/j.1749-6632.1996.tb53546.x

Ullmann L, Guimarães F, Fornazari F, Tomé R, Camossi L, Greca H, et al. Ações de vigilância continuada papel do cão como animal sentinela para toxoplasmose. Rev. Bras. Parasitol. Vet. 2008;17(Supl. 1):345-347.

Camossi LG, Faccioli PY, Menozzi BD, Daher SR, Langoni H. Environmental risk factors for canine toxoplasmosis in a deprived district of Botucatu, SP, Brazil. J. Venom. Anim. Toxins. incl. Trop. Dis. 2008;14(3):450-465. DOI: https://doi.org/10.1590/S1678-91992008000300006

Pimenta AL, Piza ET, Cardoso Jr RB, Dubey JP. Visceral toxoplasmosis in dogs from Brazil. Vet. Parasitol. 1993;45:323-326. DOI: https://doi.org/10.1016/0304-4017(93)90086-3

Bernsteen L, Gregory C, Aronson L, Lirtzman R, Brummer D. Acute toxoplasmosis following renal transplantation in three cats and a dog. J. Am. Vet. Med. Assoc. 1999;215(8):1123-126.

Mineo TWP, Silva DAO, Costa GHN, Von Ancken ACB, Kasper LH, Souza MA, et al. Detection of IgG antibodies to Neospora caninum and Toxoplasma gondii in dogs examined in a veterinary hospital from Brazil. Vet. Parasitol. 2001; 98:239-245. DOI: https://doi.org/10.1016/S0304-4017(01)00441-1

Brito AF, Souza LC, Silva AV, Langoni H. Epidemiological and serological aspects in canine toxoplasmosis in animals with nervous symptoms. Mem. Inst. Oswaldo Cruz. 2002; 97(1):31-35. DOI: https://doi.org/10.1590/S0074-02762002000100003

Moretti L, Ueno T, Ribeiro M, Aguiar D, Paes A, Pezerico S, et al. Toxoplasmose em cães coinfectados com o vírus da cinomose. Semin. Cienc. Agrar. 2002; 23(1):85-91. DOI: https://doi.org/10.5433/1679-0359.2002v23n1p85

Dubey JP, Pimenta AL, Abboud LCS, Ravasani RR, Mense M. Dermatitis in a dog associated with an unidentified Toxoplasma gondii-like parasite. Vet Parasitol. 2003;116(1):51-59. doi:10.1016/S0304-4017(03)00240-1. DOI: https://doi.org/10.1016/S0304-4017(03)00240-1

Silva R, Rolim R, Tanaka E, Langoni H, Lopes A, Rocha F, et al. Seroepidemiological evaluation of toxoplasmosis leptospirosis and canine leishmaniosis in the municipality of Ubatuba, SP. Arch. Biol. Instit. 2003; 70(3):424-428.

Freschi CR, Higa AC, Tinucci Costa M, Pancracio HP, Machado RZ. Caracterização de antígenos de Toxoplasma gondii, pela técnica de “western blotting” em soros de cães com sinais clínicos suspeitos de toxoplasmose. ARS Vet. 2005;21(2):265-271.

Sevá AP, Silva RC, Silva AV, Castro APB, Menozzi BD, Langoni H. Avaliação da virulência de cepas de Toxoplasma gondii em camundongos, isoladas de cães com sinais neurológicos, em Botucatu, SP. Vet. e Zoot. 2006;13(1):33-43.

Silva A, Gonçalves G, Livero F, Bottin J, Belinato F, Bastos Junior E, et al. Evaluation of epidemiological factors in the occurrence of antibodies against Toxoplasma gondii in dogs trested in a university hospital. Vet. e Zoot. 2009;16(1):239-247.

Moretti L d’Arc, Silva AV, Ribeiro MG, Paes AC, Langoni H. Toxoplasma gondii genotyping in a dog co-infected with distemper virus and ehrlichiosis rickettsia. Rev Inst. Med. Trop. S. Paulo. 2006;48(6):359-263. DOI: https://doi.org/10.1590/S0036-46652006000600012

Swinger RL, Schmidt KA, Dubielzig RR. Keratoconjunctivitis associated with Toxoplasma gondii in a dog. Vet. Ophthalmol. 2009; 12(1):56-60. DOI: https://doi.org/10.1111/j.1463-5224.2009.00675.x

Plugge N, Ferreira F, Richartz R, Siqueira A, Dittrich R. Occurrence of antibodies against Neospora caninum and/or Toxoplasma gondii in dogs with neurological signs. Rev. Bras. Parasitol. Vet. 2011; 20(3):202-206. DOI: https://doi.org/10.1590/S1984-29612011000300004

Hoffmann AR, Cadieu J, Kiupel M, Lim A, Bolin SR, Mansell J. Cutaneous toxoplasmosis in two dogs. J. Vet. Diag. Invest. 2012;24(3):636-640. doi: 10.1177/1040638712440995. DOI: https://doi.org/10.1177/1040638712440995

Sakamoto K, Melo G, Machado G. T and B lymphocytes in the brains of dogs with concomitant seropositivity to three pathogenic protozoans: Leishmania chagasi, Toxoplasma gondii and Neospora caninum. BMC. 2013; 6:(226). doi:10.1186/1756-0500-6-226. DOI: https://doi.org/10.1186/1756-0500-6-226

Silva MC, Fighera RA, Brum JS, Graça DL, Kommers GD, Irigoyen LF, et al. Aspéctos clinicopatológicos de 620 casos neurológicos de cinomose em cães. Pesq. Vet. Bras. 2007; 27(5):215-220. DOI: https://doi.org/10.1590/S0100-736X2007000500006

Headley SA, Alfieri AA, Fritzen JTT, Garcia JL, Weissenböck H, Silva AP, et al. Concomitant canine distemper, infectious canine hepatitis, canine parvoviral enteritis, canine infectious tracheobronchitis, and toxoplasmosis in a puppy. J. Vet. Diag. Invest. 2013;25(1):129-135. doi:10.1177/1040638712471344. DOI: https://doi.org/10.1177/1040638712471344

Dubey J, Lappin M. Infectious diseases of the dog and the cat. 3th ed. Saunders; 2006.

Sabin AB, Feldman HA. Dyes as microchemical indicators of a new immunity phenomenon affecting a protozoon parasite (Toxoplasma). Science. 1948; 108:660-663. DOI: https://doi.org/10.1126/science.108.2815.660

Dubey JP. Toxoplasmosis of Animals and Humans. 2nd ed. Boca Raton: CRC Press; 2010.

Silva AV, Cutolo AA, Langoni H. Comparação da reação de imunofluorescência indireta e do método de aglutinação direta na detecção de anticorpos anti-toxoplasma em soros de ovinos, caprinos, caninos e felinos. Arq. Inst. Biol. 2002;69(1):7-11.

Camargo M, Leser P, Guarnieri D, Rocca A. Standardization of serological tests for toxoplasmosis urgent problem of clinical pathology. Rev. Bras. Patol. Clin. 1977;13(1):1-5.

Balfour AH, Fleck DG, Hughes HPA, Sharp D. Comparative study of three tests (dye test, indirect haemagglutination test, latex agglutination test) for the detection of antibodies to Toxoplasma gondii in human sera. J. Clin. Pathol. 1982; 35(2):228-232. DOI: https://doi.org/10.1136/jcp.35.2.228

Langoni H, Matteucci G, Medici B, Camossi L, Richini-Pereira V, Silva R. Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders. Rev. Soc. Bras. Med. Trop. 2012; 45(3):365-368. DOI: https://doi.org/10.1590/S0037-86822012000300016

Coiro C, Langoni H, Silva R, Ullmann L. Fatores de risco para leptospirose leishmaniose neosporose e toxoplasmose em cães domiciliados e peridomiciliados em Botucatu. Vet. e Zoot. 2011; 18(3):393-407.

Dantas S, Fernandes A, Souza Neto O, Mota R, Alves C, Azevedo S. Ocorrence and risk factors associated with Toxoplasma gondii and Neospora caninum infections in dogs in the county of Natal Rio Grande do Norte state Northeastern Brazil. Ciênc. Rural. 2013; 43(11):2042-2048. DOI: https://doi.org/10.1590/S0103-84782013001100020

Cañón-Franco WA, Bergamaschi DP, Labruna MB, Camargo LMA, Silva JCR, Pinter A, et al. Occurrence of anti-Toxoplasma gondii antibodies in dogs in the urban area of Monte Negro, Rondônia, Brazil. Vet. Res. Commun. 2004; 28(2):113-118. DOI: https://doi.org/10.1023/B:VERC.0000012114.71235.73

Azevedo SS, Batista CSA, Vasconcellos SA, Aguiar DM, Ragozo AMA, Rodrigues AAR, et al. Seroepidemiology of Toxoplasma gondii and Neospora caninum in dogs from the state of Paraíba, Northeast region of Brazil. Res. Vet. Sci. 2005; 79(1):51-56. doi:10.1016/j.rvsc.2004.10.001. DOI: https://doi.org/10.1016/j.rvsc.2004.10.001

Gaio F, Salina A, Menozzi B, Langoni H. Anticorpos anti-Toxoplasma gondii e anti-Neospora caninum em cães da zona rural do município de Botucatu - São Paulo, Brasil. Vet. e Zoot. 2014; 21(2):288-296.

Gava MZ, Silva EC, Cunha CM, Menozzi BD, Barbosa AN, Langoni H. Infecção pelo Toxoplasma gondii em cães: soroepidemiologia e saúde pública. Brazil. J. Infect. Dis. 2021; 25(S1):101078. doi.org/10.1016/j.bjid.2020.101198. DOI: https://doi.org/10.1016/j.bjid.2020.101198

Olbera AVG, Fornazari F, Babboni SD, Rossi RS, Sevá AP, Latosinski GS, et al. Cumulative incidence and spatial distribution of dogs exposed to Toxoplasma gondii. Braz. J. Parasitol. Vet. 2020; 29(2): e000820. doi:10.1590/S1984-29612020025 DOI: https://doi.org/10.1590/s1984-29612020025

Fernandes ARF, Costa DF, Andrade MR, Bezerra CS, Mota RA, Alves CJ, et al. Seropositivity and risk factors for leptospirosis, toxoplasmosis and neosporosis in the canine population of Paraiba state, northeastern Brazil. Pesq. Vet. Bras. 2018; 38(5):957-966. doi:10.1590/1678-5150-PVB-5137 DOI: https://doi.org/10.1590/1678-5150-pvb-5137

Paz GS, Colhado BS, Anton MM, Rocha SK, Silva DB, Moraes CCG, et al. Infection by Toxoplasma gondii, Neospora caninum, Leishmania major and Trypanosoma cruzi in dogs from the state of Pará. Cienc. Anim. Bras. 2019; 20:1-10. doi:10.1590/1809-6891v20e-33566. DOI: https://doi.org/10.1590/1809-6891v20e-33566

Mantovan KB, Luiz CGS, Menozzi BD, Nilsson MG, Latosinski GS, Silva RC, et al. Pesquisa de anticorpos para Toxoplasma gondii, Leptospira spp e Leishmania spp em cães no município de Pardinho, São Paulo. Vet. e Zoot. 2021; 28:1-10.

Costa V, Silva R, Brant J, Modolo J, Langoni H. Prevalence and geographical distribution of Toxoplasma gondii in dogs in the urban area of Botucatu, SP, Brazil. Braz. J. Vet. Res. Anim Sci. 2013; 50(2):152-155. DOI: https://doi.org/10.11606/issn.2318-3659.v50i2p152-155

Langoni H, Fornazari F, Silva RC, Monti T, Villa FB. Prevalence of antibodies against Toxoplasma gondii and Neospora caninum in dogs. Braz. J. Microiol. 2013; 44(4):1327-1330. DOI: https://doi.org/10.1590/S1517-83822013000400043

Silva R, Lima V, Tanaka E, Silva A, Souza L, Langoni H. Risk factors and presence of antibodies to Toxoplasma gondii in dogs from the coast of São Paulo state Brazil. Pesq. Vet. Bras. 2010; 30(2):161-166. DOI: https://doi.org/10.1590/S0100-736X2010000200011

Langoni H, Modolo JR, Pezerico SB, Silva RC, Castro APB, Silva AV, et al. Serological profile of anti-Toxoplasma gondii antibodies in apparently healthy dogs of the city of Botucatu, São Paulo state, Brazil. J. Venom. Anim. Toxins. incl Trop. Dis. 2006; 12(1):142-148. DOI: https://doi.org/10.1590/S1678-91992006000100012

Published

2023-12-14

How to Cite

1.
Moschen Batista AC, Angeluci GC, Jabur Lot Rodrigues N, dos Santos Rocha G, Gonçalves Lima S, Ribeiro Braga F, Hiura E, de Barros LD, Vieira da Silva A, Menozzi BD, Langoni H. EVALUATION OF CANINE TOXOPLASMOSIS SEROPREVALENCE IN ANIMALS FROM GREATER VITÓRIA-ES. RVZ [Internet]. 2023 Dec. 14 [cited 2024 Nov. 24];30:1-10. Available from: https://rvz.emnuvens.com.br/rvz/article/view/1569

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