Fractionated collection and their main benefits in the stallion sperm cryopreservation
DOI:
https://doi.org/10.35172/rvz.2017.v24.281Keywords:
equine, fractionated collection, semenAbstract
Equine semen is collected by conventional way, with a closed artificial vagina, where all the
fractions are mixed. However, semen collection discontinuously, separating the different
fractions of the ejaculate, each of these fractions have a greater or lesser amount of seminal
plasma, being the sex accessory glands responsible for their production. Each stud has
individual characteristics with respect to the maintenance of sperm cells during the
cryopreservation, these characteristics may be related to production of seminal plasma as well
as the various components making up. It is known that there is a deleterious effect played by
components of seminal plasma mainly produced by the seminal vesicles, the sperm cells
during the cryopreservation process, however, these factors are still unknown. Several
hypotheses are suggested such as reducing the production of lipids and proteins and the types
of ions present in each fraction of the ejaculate. Knowing this, the fractional harvest can be
beneficial to sperm, so that the first fraction have no contact with seminal plasma produced by
the seminal vesicles, allowing for better maintenance sperm during cryopreservation.
References
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seminal plasma on sperm longevity. Anim Reprod Sci. 2008;10:249-56.
2. Kareskoski AM, Reilas T, Andersson M, Katila T. Motility and plasma membrane
integrity of spermatozoa in fractionated stallion ejaculates after storage. Reprod Domest
Anim. 2006;41:33-8.
3. Schembri MA, Major DA, Suttie JJ, Maxwell WM, Evans G. Capacitation-like changes in
equine spermatozoa throughout the cryopreservation process. Reprod Fertil Dev.
2002;14:225-33.
4. Alghamdi AS, Troedsson MH, Xue JL, Crabo BG. Effect of seminal plasma concentration
and various extenders on postthaw motility and glass wool-Sephadex filtration of
cryopreserved stallion semen. Am J Vet Res. 2002;63:880-5.
5. Jasko DJ, Hathaway JA, Schaltenbrand VL, Simper WD, Squires EL. Effect of seminal
plasma and egg yolk on motion characteristics of cooled stallion spermatozoa.
Theriogenology. 1992;37:1241-52.
6. Akcay E, Reilas T, Andersson M, Katila T. Effect of seminal plasma fractions on sperm
survival after cooled storage. J Vet Med A Physiol Pathol Clin Med. 2006;53:481-5.
7. Katila T, Reilas T, Guvenc K, Alm K, Andersson M. The effect of seminal plasma on
motility characteristics and viability of spermatozoa after cooled storage. In: Proceedings
of the 3rd Meeting of the EEGG; 2004; Suffolk, UK. Suffolk: EEGG; 2004. p.3-5.
8. Sieme H, Katila T, Klug E. Effect of semen collection practices on sperm characteristics
before and after storage and on fertility of stallions. Theriogenology. 2004;61:769-84.
9. Varner DD, Blanchard TL, Love CL, Garcia MC, Kenney RM. Effects of semen
fractionation and dilution ratio on equine spermatozoal motility parameters.
Theriogenology. 1987;28:709-18.
10. Crump Jr J, Crump J. Stallion ejaculation induced by manual stimulation of the penis.
Theriogenology. 1989;31:341-6.
11. McDonnell SM, Love CC. Manual stimulation collection of semen from stallions:
training time, sexual behavior and semen. Theriogenology. 1990;33:6.
12. McDonnell SM. Ejaculation physiology and dysfunction. Vet Clin North Am Equine
Pract. 1992;8:57-70.
13. McDonnell SM. Techniques for extending the breeding career of aging and disabled
stallions. Clin Tech Equine Pract. 2005;4:269-76.
14. Berliner V. Horses and Jackstock. In: Perry EJ. The artificial insemination of farm
animals. New Brunswick, NJ: Rutgers University Press; 1960.
15. Schumacher J, Riddell MG. Collection of stallion semen without a mount.
Theriogenology. 1986;26:245-50.
16. McKinnon AO, Voss JL. Equine reproduction. Philadelphia: Lea and Febiger; 1992.
17. Fonseca CW. Efeito de uma nova técnica de coleta fracionada sobre a composição do
plasma seminal e qualidade do sêmen equino pós-descongelamento [dissertação].
Campos dos Goytacazes: Universidade estadual do norte fluminense Darcy Ribeiro;
2006.
18. Lopes MD, Papa FO, Martino AP, Bicudo SD, Alvarenga MA. Efeito do tipo de mucosa
de vagina artificial sobre a qualidade espermática de garanhões. Rev Bras Reprod Anim.
1997;21:29-34.
19. Tischner M, Kosiniak K, Bielanski W. Analysis of the pattern of ejaculation in stallions. J
Reprod Fertil. 1974;41:329-35.
20. Rodríguez-Martínez H, Kvist U, Ernerudh J, Sanz L, Calvete JJ. Seminal plasma
proteins: what role do they play? Am J Reprod Immunol. 2011;66:11-22.
21. Tischner M, Kosiniak K. Techniques for collection and storage of stallion semen with
minimal secondary contamination. Acta Vet Scand. 1992;88:83-90.
22. Silva KMG, Papa FO, Gabaldi SH, Crocci AJ. Efeito dos antibióticos gentamicina e
cefalotina e do aminoácido taurina em meio de gema de ovo (Baken), sobre a
longevidade e fertilidade do sêmen resfriado de equino. Rev Bras Cienc Vet. 2003;10:81-
7.
23. McDonnell SM. Oral imipramine and intravenous xylazine for pharmacologicallyinduced ex copula ejaculation in stallions. Anim Reprod Sci. 2001;68:153-9.
24. Papa FO, Melo CM, Fioratti EG, Dell’Aqua Jr. JA, Zahn FS, Alvarenga MA. Freezing of
stallion epididymal sperm. Anim Reprod Sci. 2008;107:293-301.
25. Heise A, Thompson PN, Gerber D. Influence of seminal plasma on fresh and post-thaw
parameters of stallion epididymal spermatozoa. Anim Reprod Sci. 2011;123:192-201.
26. Monteiro GA, Papa FO, Zahn FS, Dell’Aqua Jr. JA, Melo CM, Maziero RRD, et al.
Cryopreservation and fertility of ejaculated and epididymal stallion Sperm. Anim Reprod
Sci. 2011;127:197-201.
27. Monteiro GA, Guasti PN, Rocha AS, Martin I, Sancler-Silva YFR, Freitas Dell’Aqua CP,
et al. Effect of storage time and temperature of equine epididymis on the viability, motion
parameters, and freezability of epididymal sperm. J Equine Vet Sci. 2013;33:169-73
28. Mann T, Short RV, Walton A. The 'tail-end sample' of stallion semen. J Agric Sci
Cambridge. 1957;49:301.
29. Lindeberg H, Karjalainen H, Koskinen E, Katila T. Quality of stallion semen obtained by
a new semen collection phantom (equidame®) versus a missouri® artificial vagina.
Theriogenology. 1999;51:1157-73.
30. Kareskoski AM. Components of fractionated stallion seminal plasma and the effects of
seminal plasma on sperm longevity [dissertação]. Helsinki: Faculty of Veterinary
Medicine, University of Helsinki Finland; 2011.
31. Oliveira SN, Araujo EAB, Chaves-Silva LFM, Andrade Jr LRP, Dalanezi FM, Pinto BM,
et al. Eficiência de uma nova técnica de coleta de sêmen para diagnóstico e controle de
piospermia em garanhões: relato de caso. In: Anais da 16a Conferência Anual da
ABRAVEQ; 2015; Águas de Lindóia. Águas de Lindóia: ABRAVEQ; 2015.
32. Oliveira SN, Andrade Jr. LRP, Chaves-Silva LFM, Araujo EAB, Dalanezi FM, Papa PM,
et al. Novo método de colheita fracionada de sêmen para melhoria da fertilidade em
garanhão com vesiculite seminal. In: Anais da 17a Conferência Anual da ABRAVEQ;
2016; Campos do Jordão. Campos do Jordão: ABRAVEQ; 2016
seminal plasma on sperm longevity. Anim Reprod Sci. 2008;10:249-56.
2. Kareskoski AM, Reilas T, Andersson M, Katila T. Motility and plasma membrane
integrity of spermatozoa in fractionated stallion ejaculates after storage. Reprod Domest
Anim. 2006;41:33-8.
3. Schembri MA, Major DA, Suttie JJ, Maxwell WM, Evans G. Capacitation-like changes in
equine spermatozoa throughout the cryopreservation process. Reprod Fertil Dev.
2002;14:225-33.
4. Alghamdi AS, Troedsson MH, Xue JL, Crabo BG. Effect of seminal plasma concentration
and various extenders on postthaw motility and glass wool-Sephadex filtration of
cryopreserved stallion semen. Am J Vet Res. 2002;63:880-5.
5. Jasko DJ, Hathaway JA, Schaltenbrand VL, Simper WD, Squires EL. Effect of seminal
plasma and egg yolk on motion characteristics of cooled stallion spermatozoa.
Theriogenology. 1992;37:1241-52.
6. Akcay E, Reilas T, Andersson M, Katila T. Effect of seminal plasma fractions on sperm
survival after cooled storage. J Vet Med A Physiol Pathol Clin Med. 2006;53:481-5.
7. Katila T, Reilas T, Guvenc K, Alm K, Andersson M. The effect of seminal plasma on
motility characteristics and viability of spermatozoa after cooled storage. In: Proceedings
of the 3rd Meeting of the EEGG; 2004; Suffolk, UK. Suffolk: EEGG; 2004. p.3-5.
8. Sieme H, Katila T, Klug E. Effect of semen collection practices on sperm characteristics
before and after storage and on fertility of stallions. Theriogenology. 2004;61:769-84.
9. Varner DD, Blanchard TL, Love CL, Garcia MC, Kenney RM. Effects of semen
fractionation and dilution ratio on equine spermatozoal motility parameters.
Theriogenology. 1987;28:709-18.
10. Crump Jr J, Crump J. Stallion ejaculation induced by manual stimulation of the penis.
Theriogenology. 1989;31:341-6.
11. McDonnell SM, Love CC. Manual stimulation collection of semen from stallions:
training time, sexual behavior and semen. Theriogenology. 1990;33:6.
12. McDonnell SM. Ejaculation physiology and dysfunction. Vet Clin North Am Equine
Pract. 1992;8:57-70.
13. McDonnell SM. Techniques for extending the breeding career of aging and disabled
stallions. Clin Tech Equine Pract. 2005;4:269-76.
14. Berliner V. Horses and Jackstock. In: Perry EJ. The artificial insemination of farm
animals. New Brunswick, NJ: Rutgers University Press; 1960.
15. Schumacher J, Riddell MG. Collection of stallion semen without a mount.
Theriogenology. 1986;26:245-50.
16. McKinnon AO, Voss JL. Equine reproduction. Philadelphia: Lea and Febiger; 1992.
17. Fonseca CW. Efeito de uma nova técnica de coleta fracionada sobre a composição do
plasma seminal e qualidade do sêmen equino pós-descongelamento [dissertação].
Campos dos Goytacazes: Universidade estadual do norte fluminense Darcy Ribeiro;
2006.
18. Lopes MD, Papa FO, Martino AP, Bicudo SD, Alvarenga MA. Efeito do tipo de mucosa
de vagina artificial sobre a qualidade espermática de garanhões. Rev Bras Reprod Anim.
1997;21:29-34.
19. Tischner M, Kosiniak K, Bielanski W. Analysis of the pattern of ejaculation in stallions. J
Reprod Fertil. 1974;41:329-35.
20. Rodríguez-Martínez H, Kvist U, Ernerudh J, Sanz L, Calvete JJ. Seminal plasma
proteins: what role do they play? Am J Reprod Immunol. 2011;66:11-22.
21. Tischner M, Kosiniak K. Techniques for collection and storage of stallion semen with
minimal secondary contamination. Acta Vet Scand. 1992;88:83-90.
22. Silva KMG, Papa FO, Gabaldi SH, Crocci AJ. Efeito dos antibióticos gentamicina e
cefalotina e do aminoácido taurina em meio de gema de ovo (Baken), sobre a
longevidade e fertilidade do sêmen resfriado de equino. Rev Bras Cienc Vet. 2003;10:81-
7.
23. McDonnell SM. Oral imipramine and intravenous xylazine for pharmacologicallyinduced ex copula ejaculation in stallions. Anim Reprod Sci. 2001;68:153-9.
24. Papa FO, Melo CM, Fioratti EG, Dell’Aqua Jr. JA, Zahn FS, Alvarenga MA. Freezing of
stallion epididymal sperm. Anim Reprod Sci. 2008;107:293-301.
25. Heise A, Thompson PN, Gerber D. Influence of seminal plasma on fresh and post-thaw
parameters of stallion epididymal spermatozoa. Anim Reprod Sci. 2011;123:192-201.
26. Monteiro GA, Papa FO, Zahn FS, Dell’Aqua Jr. JA, Melo CM, Maziero RRD, et al.
Cryopreservation and fertility of ejaculated and epididymal stallion Sperm. Anim Reprod
Sci. 2011;127:197-201.
27. Monteiro GA, Guasti PN, Rocha AS, Martin I, Sancler-Silva YFR, Freitas Dell’Aqua CP,
et al. Effect of storage time and temperature of equine epididymis on the viability, motion
parameters, and freezability of epididymal sperm. J Equine Vet Sci. 2013;33:169-73
28. Mann T, Short RV, Walton A. The 'tail-end sample' of stallion semen. J Agric Sci
Cambridge. 1957;49:301.
29. Lindeberg H, Karjalainen H, Koskinen E, Katila T. Quality of stallion semen obtained by
a new semen collection phantom (equidame®) versus a missouri® artificial vagina.
Theriogenology. 1999;51:1157-73.
30. Kareskoski AM. Components of fractionated stallion seminal plasma and the effects of
seminal plasma on sperm longevity [dissertação]. Helsinki: Faculty of Veterinary
Medicine, University of Helsinki Finland; 2011.
31. Oliveira SN, Araujo EAB, Chaves-Silva LFM, Andrade Jr LRP, Dalanezi FM, Pinto BM,
et al. Eficiência de uma nova técnica de coleta de sêmen para diagnóstico e controle de
piospermia em garanhões: relato de caso. In: Anais da 16a Conferência Anual da
ABRAVEQ; 2015; Águas de Lindóia. Águas de Lindóia: ABRAVEQ; 2015.
32. Oliveira SN, Andrade Jr. LRP, Chaves-Silva LFM, Araujo EAB, Dalanezi FM, Papa PM,
et al. Novo método de colheita fracionada de sêmen para melhoria da fertilidade em
garanhão com vesiculite seminal. In: Anais da 17a Conferência Anual da ABRAVEQ;
2016; Campos do Jordão. Campos do Jordão: ABRAVEQ; 2016
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Published
2017-09-29
How to Cite
1.
Nunes de Oliveira S, Adonis Braga de Araujo E, Mercês Chaves Silva LF, Morales Dalanezi F, de Mello Papa P, Moreira Pinto B, Pena de Andrade Junior LR, Ozanam Papa F. Fractionated collection and their main benefits in the stallion sperm cryopreservation. RVZ [Internet]. 2017 Sep. 29 [cited 2024 Dec. 17];24(3):435-43. Available from: https://rvz.emnuvens.com.br/rvz/article/view/281
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