Laser therapy for treatment of tendinopathy induced in wistar rats
An histomorfometric study
DOI:
https://doi.org/10.35172/rvz.2017.v24.290Keywords:
Achilles tendon, low-level laser therapy, tendon healingAbstract
The aim of this study was to evaluate the effects of laser therapy in tendinopathy of Achilles
tendon of Wistar rats. Thus, 12 adult male rats which were divided into two groups (L=laser
and C=control) were used. All animals were submitted to unilateral tendinopathy (random
selection) by transverse compression of the tendon (10 seconds) with Halsted forceps, as well
as 10 scarifications (using a scalpel). After 24 hours of induction of lesion all animals of
Group L received laser (904 nm/3 J/cm²/ 9 s) for 20 days, while Group C were handled as if
they would receive radiation. After 3, 7, 14 and 21 days lesion induction, three rats from each
group were euthanized, and the tendons obtained for histomorphometric analysis. The
samples were processed as routine and the sections stained with hematoxylin-eosin, Masson's
trichrome and Picrosirius Red. Data were analyzed by ANOVA, at 5% probability and
regression analysis. No differences between groups neither between time for hemorrhage
characteristics, angiogenesis, thickening of epitendon. Independently of the treatment there
was a decrease (p=0.0129) of fibrinous adhesion (3rd to 21st day). On the other hand,
morphometric analysis revealed higher (p=0.0120) amounts of fibroblasts cells in the group
receiving laser therapy, with no effect of time. Semiquantitative assessment also showed
higher (p=0.0000) amounts of fibroblasts cells in the treated group, but in this analysis, the
number of fibroblasts increased with time (p=0.0001) in both group. In contrast, ANOVA
revealed a reduction of the inflammatory cells from 3rd to 21st day in both groups (histology:
p=0.0003; morphometry: p=0.0000). Although there was no difference between groups in the
amount of collagen type I and III, morphometry revealed that the rats of group L showed
higher (p=0.0096) amounts of collagen fibers than group C. For this characteristic, there was
no effect of time, even though it was observed higher (p=0.0000) organization of collagen
deposition on day 7. The amount of collagen fibers determined by histology revealed only the
effect of time (p=0.0005), regardless of treatment, with an increase in this variable until the
14th day, with further reduction. Laser therapy initiated 24 hours after surgical induction of
tendinopathy in the Achilles tendon of rats has the advantage of increasing quality and
quantity collagen fibers, as well as the fibroblasts cells, which are fundamental on the
synthesis of collagen.
References
2007;41(4):232-40.
2. Andres BM, Murrell GAC. Treatment of tendinopathy: what works, what does not and
what is on horizon. Clin Orthop Relat Res. 2008;466(7):1539-54.
3. Medeiros UVD, Segatto GG. Lesões por esforços repetitivos (LER) e distúrbios
osteomusculares (Dort) em dentistas. Rev Bras Odontol. 2012;69(1):49-54.
4. Fillipin L, Mauriz JL, Vedoveli K, Moreira AJ, Zettler ZG, Lech O, et al. Low-level laser
therapy (LLLT) prevents oxidative stress and reduces fibrosis in rat traumatized Achilles
tendon. Lasers Surg Med. 2005;37(4):293-300.
5. Wang JH-C. Mechanobiology of tendon. J Biomech. 2006;39(9):1563-82.
6. Dahlgren LA. Pathobiology of tendon and ligament injuries. Clin Tech Equine Pract.
2007;6(3):168-73.
7. Souza MV, Pinto JO. Association between nonsteroidal anti-inflammatory drugs and
gastric ulceration in horses and ponies. In: Jianyuan C. Peptic ulcer disease. Rijeka:
Intech; 2011. p.463-86.
8. Salate ACB, Barbosa G, Gaspar P, Koeke PU, Parizotto NA, Benze BG, et al. Effect of
In-Ga-Al-P Diode laser irradiation on angiogenesis in partial ruptures of Achilles tendon
in rats. Photomed Laser Surg. 2005;23(5):470-5.
9. Moreira FF, Oliveira ELP, Barbosa FS, Silva JG. Laserterapia de baixa intensidade na
expressão de colágeno após lesão muscular cirúrgica. Fisioter Pesqui. 2011;18(1):37-42.
10. Pufe T, Petersen WJ, Mentlein R, Tillmann BN. The role of vasculature and
angiogenesis of degenerative tendons disease. Scand J Med Sci Sports. 2005;15(4):211-
22.
11. Silva MO, Costa MBM, Borges APB, Dornas RF, Moreira JCL, Souza MV. Indução de
tendinopatia em ratos Wistar: modelo experimental. Rev Acad Cienc Agrar Ambient.
2013;11(3):275-82.
12. Orhan Z, Ozturan K, Guven A, Cam K. The effect of extracorporeal shock waves on a rat
model of injury to tendo Achillis: a histological and biomechanical study. J Bone Joint
Surg Br. 2004;86(4):613-8.
13. Eliasson P, Andersson T, Aspenberg P. Achilles tendon healing in rats is improved by
intermittent mechanical loading during the inflammatory phase. J Orthop Res.
2012;30(2):274-9.
14. Van Schie HT, Bakker EM, Jonker AM, Van Weeren PR. Computerized
ultrasonographic tissue characterization of equine superficial digital flexor tendons by
means of stability quantification of echo patterns in contiguous transverse
ultrasonographic images. Am J Vet Res. 2003;64(3):366-75.
15. Abate M, Gravare-Silbernagel K, Siljeholm C, Di Lori A, De Amicis D, Salini V, et al.
Pathogenesis of tendinopathies: inflammation or degeneration? Arthritis Res Ther.
2009;11(3):1-15.
16. Sharma P, Maffulli N. Tendon injury and tendinopathy: healing and repair. J Bone Joint
Surg Am. 2005;87(1):187-202.
17. James R, Kesturu G, Balian G, Chhabra AB. Tendon: biology, biomechanics, repair,
growth, factors and envolving treatment options. J Hand Surg Am. 2008;33A(1):102-12.
18. Laraia SEM, Silva IS, Pereira DM, Reis FA, Almeida P, Leal Júnior ECP, et al. Effect of
low-level laser therapy (660 nm) on acute inflammation induced by tenotomy of Achilles
tendon in rats. Photochem Photobiol. 2012;88(6):1546-50.
19. Pohlers D, Brenmoehl J, Loffer I, Muller CK, Leipner C, Schultze-Mosgau S, et al.
TGF-β and fibrosis in different organs molecular pathway imprints. Biochim Biophys
Acta. 2009;1792(8):746-56.
20. McGrath MH. Peptide growth factors and wound healing. Clin Plast Surg.
1990;17(3):421-32.
21. Branton MH, Kopp JB. TGF-â and fibrosis. Microbes Infect. 1999;1(15):1349-65.
22. Klein MB, Yalamanchi N, Pham H, Longaker MT, Chang J. Flexor tendon healing in
vitro: effect of TGF-β on tendon cell collagen production. J Hand Surg Am.
2002;27(4):615-20.
23. Marcos RL. Efeito do laser de baixa potência (810 nM) na tendinite induzida por
colagenase em tendão calcâneo de ratos [tese]. São Paulo: Universidade de São Paulo;
2010.
24. Pires D, Xavier M, Araujo T, Silva Júnior JA, Aimbre F, Albertini R. Low-level laser
therapy (LLLT; 780 nm) acts differently on mRNA expression of anti- and proinflammatory mediators in an experimental model of collagenase-induced tendinitis in
rat. Lasers Med Sci. 2011;26(1):85-94.
25. Xavier M, de Souza RA, Pires VA, Santos AP, Aimbire F, Silva JA Jr, et al. Low-level
light-emitting diode therapy increases mRNA expressions of IL-10 and type I and III
collagens on Achilles tendinitis in rats. Lasers Med Sci. 2014;29(1):85-90.
26. Nakamura K, Kitaoka K, Tomita K. Effect of eccentric exercise on healing process of
injured patellar tendon in rats. J Orthop Sci. 2008;13(4):371-8.
27. Bjordal JM, Lopes-Martins RAB, Joensen J, Couppe C, Ljunggren AE, Stergioulas A, et
al. A systematic review with procedural assessments and meta-analysis of low level laser
therapy in lateral elbow tendinopathy (tennis elbow). BMC Musculoskelet Disord.
2008;9(75):1-15.
28. Lins RDAU, Dantas EM, Lucena KCR, Catão MHCV, Granville-Garcia AF, Carvalho
Neto LG. Efeitos bioestimulantes do laser de baixa potência no processo de reparo. An
Bras Dermatol. 2010;85(6):849-55.
29. Lui PPY, Cheuk YC, Hung LK, Fu SC, Chan KM. Increased apoptosis at the late stage of
tendon healing. Wound Repair Regen. 2007;15(5):702-7.
30. Guerra FR, Vieira CP, Almeida MS, Oliveira LP, Aro AA, Pimentel ER. LLLT improves
tendon healing through increase of MMP activity and collagen synthesis. Lasers Med
Sci. 2013;28(5):1281-8.
31. Medrado ARAP, Pugliese LS, Reis SRA, Andrade ZA. Influence of low level laser
therapy on wound healing and its biological action upon myofibroblasts. Lasers Surg
Med. 2003;8(3):239-44.
32. Barbosa D, Souza RA, Carvalho WRG, Xavier M, Carvalho PK, Cunha CR, et al. Lowlevel laser therapy combined with platelet-rich plasma on the healing calcaneal tendon: a
histological study in a rat model. Lasers Med Sci. 2013;28(6):1489-94.
33. Kajikawa Y, Morihara T, Sakamoto H, Matsuda K, Oshima Y, Yoshida A, et al. Plateletrich plasma enhances the initial mobilization of circulation-derived cells for tendon
healing. J Cell Physiol. 2008;215(3):837-45.
34. Barbato KBG. Efeito do uso de antiinflamatório e do exercício aeróbico sobre a
regeneração tecidual e perfil biomecânico do tendão calcâneo de ratos após ruptura
completa [tese]. Rio de Janeiro: Universidade do Estado do Rio de Janeiro; 2011.
35. Silva MO. Efeitos da laserterapia de baixa potência associada ou não a exercício
excêntrico no tratamento de tendinopatia induzida do tendão calcanear comum de ratos
(Rattus norvegicus) [dissertação]. Viçosa: Universidade Federal de Viçosa; 2013.
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