Density gradient centrifugation

Alternative for sexing sperm in dogs?

Abstract

The technical progress concerning sperm sexing in recent years has enabled significant advances, but still none method was used successfully in dogs. Despite the flow cytometry to be an effective method for sperm sexing, in dogs it can be considered difficult to implement in view of high cost of application of the technique and low sperm recovery after separation. In contrast, density gradient centrifugation can also be used for separating sperm in dogs. This technique is also based on the DNA content difference between sperm with chromosomes X and Y. Sperm separation can be confirmed by various methods such as flow cytometry, fluorescence in situ hybridization (FISH) staining with quinacrine mustard and PCR. The objective of this review to address the density gradient centrifugation as an alternative to sperm sexing in dogs