GONADOTHOPIN RELEASING HORMONE (GNRH) AND EQUINE CHORIONIC GONADOTROPIN (ECG) IMPROVE THE PREGNANCY RATE ON PROTOCOLS FOR TIMED-ARTIFICIAL INSEMINATION IN BEEF CATTLE
Keywords:
fixed-time artificial insemination, beef cattle, estrus synchronization, GnRH, eCGAbstract
The study aimed to verify the influence of GnRH and eCG in TAI protocols to improve the pregnancy rate (PR) in beef cattle, treated with previous intravaginal progesterone long-acting, in the breeding season 90 days. A total of 660 females were divided into five groups: (TAI1) injection of 1.0mg estradiol benzoate (EB) and P4 on d0, P4 removal and 0.075mg prostaglandin F2α on d9, 1.0mg EB on d10, and TAI 32h (n = 120); (TAI2) same as TAI1, but inclusion of 500μg GnRH at TAI (n = 120); (TAI3) same as TAI1, but inclusion of 400IU eCG on d10 (n = 120); (TAI4) same as TAI3, but inclusion of 500μg GnRH at TAI (n = 120); (5) no estrus synchronization, but served by natural service (NS; n = 180). Forty-five days after the TAI, the TAI1, TAI2, TAI3, and TAI4 group were run with “clean-up” bulls for 45 d. Pregnancy was determined by ultrasonography on d45 for TAI PR and on d120 after TAI for all treatments for overall PR. There were no differences in PR among the four TAI protocols on d45 (cows: TAI1=48.0%, TAI2=53.8%, TAI3=57.6%, TAI4=58.3%; heifers: TAI1=53.3%, TAI2=57.1%, TAI3=57.1%, TAI4=56.2%). The PR after the 90-day breeding season was greater in all TAI groups of cows (P<0.0001) compared with NS, except for heifers (P>0.05). Cows and heifers were analyzed together, and no differences among groups were found. It was concluded that protocols for TAI using GnRH or eCG alone or together contribute significantly to improve the pregnancy rate in beef cattle females, when subjected to previous treatment with progesterone in the reproductive season.
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