CRITERIA FOR SELECTION OF LYTIC BACTERIOPHAGE FOR USE IN MEDICAL FIELD (SETTING): PRELIMINARY DATA
Keywords:
Bacteriófagos, Salmonella, controle biológico, biotecnologiaAbstract
This article analyzed, in vitro, the behavior of 62 bacteriophages samples lytic for Salmonella
Enteritidis. The lytic potential was assessed by analyzing following parameters: proliferation,
halo size in gel diffusion, superficial halo size and virulence degree. Proliferation degree of
samples was determined by quantifying the amount of plaque forming unit (PFU) using gel
diffusion technique. To determine UFP, minor dilution with measurable halos was considered.
Lytic spectrum in the gel column was measured with an acrylic precision ruler. For to
superficial halo size and virulence degree, the “spot-on-lawn” technique was employed. We
observed that lytic activity spectrum, halo size in gel diffusion and proliferation are factors
independent of virulence, but prolific samples shown higher spectrum of lytic activity.
Prolific samples that present a high degree of virulence demonstrate in vitro a good potential
for medical application.
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