INFLUENCE OF THE ESTROUS CYCLE BITCHES IN DIAMETER OOCYTE
Keywords:
oocyte, bitches, diameter, estrous cycleAbstract
The low meiotic competence of canine oocytes when subjected to conditions of artificial
cultive is considered a major obstacle to the development of reproductive biotechnologies in
this species. Low rates of IVM may be linked to the size of oocytes recovered from different
phases of the estrous cycle. The objective of this study is to evaluate the relationship between
the phases of the estrous cycle - luteal phase and anestrus, with oocyte diameter of bitches. A
total of 41 female dogs, aged 6 months to 7 years, subject to ovariosalpingohisterectomy
elective in the Clinic of Small Animal Reproduction, Department of Animal Reproduction
and Veterinary Radiology, School of Veterinary Medicine and Animal Science, UNESP,
Botucatu. Ovaries were sliced and selected only those COCs grade I, being subjected to the
solution of 2% hyaluronidase to remove cumulus cells. After this process, the oocytes were
stained with 10 / ml of Hoechst 33342 for assessment of oocyte diameter without the zona
pellucida. The results showed no statistically significant difference between the oocytes from anestrous and luteal phases (77.62 µm x 78.64 µm ). Thus, there is a need for further studies
to be able to have a proper understanding of the factors that may be interfering with oocyte
competence and should not be considered only a determining factor since the coordinated
action of several factors contributing to the growth and developing oocyte.
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