PROTOCOL OF ISOLATION OF EQUINE MONONUCLEAR CELLS FROM BONE MARROW

Authors

  • Ana Liz Garcia Alves
  • Michelle Elisa Meletti Vieira
  • Anna Paula Balesdent Barreira
  • Ligia Souza Lima Silveira da Mota
  • Mere Erika Saito
  • Aguemi Kohayagawa
  • Carlos Alberto Hussni
  • Marcos Jun Watanabe
  • Patrícia Galvão Gomes de Oliveira

Keywords:

mononuclear fraction, stem cell, aspiration, bone marrow, equine

Abstract

In adults, the stem cells are found at the bone marrow and their isolation could be accessed by aspiration technique. This experiment aimed to evaluate the bone marrow aspiration technique and define a protocol for isolation of equine mononuclear cells. Twelve horses were submitted to bone marrow aspiration. After isolation of mononuclear cells by density gradient, the cell viability was determined obtaining a media of 86.9% of viable cells. According to the more knowledge obtained, the stem cell harvest experiment and clinical approach can be implemented in this specie.

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Published

2023-03-09

How to Cite

1.
Alves ALG, Vieira MEM, Barreira APB, Mota LSLS da, Saito ME, Kohayagawa A, Hussni CA, Watanabe MJ, Oliveira PGG de. PROTOCOL OF ISOLATION OF EQUINE MONONUCLEAR CELLS FROM BONE MARROW. RVZ [Internet]. 2023 Mar. 9 [cited 2024 Nov. 23];16(4):650-5. Available from: https://rvz.emnuvens.com.br/rvz/article/view/1264

Issue

Vol. 16 No. 4 (2009)

Section

Original Articles

License

Licença Creative Commons
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional.

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