SENSITIVITY PROFILE OF Salmonella enterica serovar Enteritidis ISOLATED FROM CHICKENS, FACED WITH DRUGS AND ANTIMICROBIAL SUBSTANCES PRODUCED BY Lactobacillus reuteri AND Lactobacillus salivarius.

Abstract

The present work verified the sensitivity and resistance profile of 15 samples of Salmonella enterica serovar Enteritidis (SE) faced with drugs and antimicrobial substances produced by Lactobacillus reuteri and Lactobacillus salivarius, and detection of antimicrobial resistance gene (integron class 1) utilizing the polymerase chain reaction technique (PCR). Streptomycin, ciprofloxacin and ampicillin presented 100% efficiency against all samples, while enrofloxacin and tetracycline showed the lowest percentages (66.7% and 60%). The highest sample resistance was observed for tetracycline (40%) and the lowest for trimethoprin (6,6%) and cefaclor (6,6%). The sensitivity of 15 SE samples faced with antimicrobial substances produced by L. salivarius was 100% in the “spot on the lawn” antagonism method, with inhibition zones varying between 16 and 24mm, while 86.7% samples (13) were sensitive to L. reuteri forming inhibition zones of about 14 to 22mm and two (13.3%) were resistant. The simultaneous antagonism method of diffusion into wells, utilizing free Lactobacillus supernatant, verified antagonistic action indicative of substances of a protein nature, in which the sensitivity and resistance of L. reuteri faced SE were 46.7% and 53.3% respectively, with inhibition zones between 13 and 15mm while L. salivarius presented 33.4% sensitivity and resistance index around 66.6%. The PCR products of SE samples amplified for the antimicrobial resistance gene sequence (integron class 1), did not present agarose gel band correspondent to amplification of the genome segment (900pb), then there was no relation between the presence of antimicrobial resistance gene and SE resistance against used drugs.