IN VITRO INHIBITION OF Salmonella spp. ISOLATED FROM CALVES OF THE SERTÃO OF ALAGOAS WITH ETHANOLIC EXTRACTS OF PLANTS FROM THE MATA ATLANTICA

Authors

  • Artur Bibiano de Vasconcelos Universidade Estadual Paulista (UNESP) https://orcid.org/0000-0001-8753-0597
  • Vitória de Andrade Universidade Federal de Alagoas
  • Danilo Alves de França Universidade Estadual Paulista (UNESP) https://orcid.org/0000-0003-1178-5643
  • Alex Alves Dantas Universidade Federal de Alagoas
  • Thiago Barros Correia da Silva Universidade Federal de Alagoas
  • Karla Patrícia Chaves da Silva Universidade Federal de Alagoas

DOI:

https://doi.org/10.35172/rvz.2022.v29.788

Keywords:

phytoconstituents, salmonellosis, public health, antimicrobial resistance, medicinal plants

Abstract

The objective of the study was to verify the in vitro inhibition of Salmonella spp. to different types of plant extracts with medicinal indications in different ethanol dilutions. For this, 12 plant parts were obtained, among leaves and stems, which are part of the popular therapeutics of gastrointestinal toxinfections in the mesoregion of Alagoas, Brazil, and that are present in the Mata Atlântica. These plants were submitted to drying, trituration, maceration and pressing procedures, allowing different extract concentrations. The Salmonella spp. samples tested came from calves from rural properties in the region that presented with diarrhea and were duly detected by biochemical and molecular tests. To verify sensitivity, the Disc Diffusion Plates test was performed with three strains of Salmonella spp., and the discs with antibiotics were replaced by discs of ethanolic extracts at concentrations of 10, 100, 500 and 1000mg/L. As a result, the leaf of Varronia multispicata, popularly known as Cavarucaá, showed positive results in inhibiting the three isolates of Salmonella spp. at concentrations of 500 and 1000mg/L. The other extracts did not show in vitro sensitivity results with the bacteria in any of the concentrations. Further studies with Varronia multispicata need to be developed, with different methods of obtaining phytoconstituents and with other strains of Salmonella spp. from the region.

References

Brasil. Ministério da Agricultura, Pecuária e Abastecimento - MAPA. Diagnóstico situacional do PNCEBT: Programa Nacional de Controle e Erradicação da Brucelose e Tuberculose Animal. Brasília, 2020.

Florindo TJ, Medeiros GIB, Costa JS, Ruviaro CF. Competitividade dos principais países exportadores de carne bovina no período de 2002 a 2013. Rev Econom Agronegócio. 2015;12:1-3.

Assis-Brasil ND, Marcolongo-Pereira C, Hinnah FL, Ladeira SRL, Sallis ESV, Grecco FB, Schild AL. Enfermidades diagnosticadas em bezerros na região sul do RS. Pesq Vet Bras. 2013;33:423-430. DOI: https://doi.org/10.1590/S0100-736X2013000400002

Strockbine NA, Bopp CA, Fields PI. Escherichia, Shigella, and Salmonella. In: Jorgensen JH, Pfaller MA (Eds.). Manual of Clinical Microbiology. 11.ed. Washington: ASM Press. 2015;685-713. DOI: https://doi.org/10.1128/9781555817381.ch37

Vasconcelos AB, Andrade V, Moraes ACI, Ramos EMOA, Silva ACA, França DA. Vasconcelos CB, Okamoto AS, Silva KPC. Ocorrência de Salmonella spp. em bezerros e perfil de resistência antimicrobiana na Mesorregião do Sertão de Alagoas, Brasil. Acta Vet Bras. 2021;15:36-40. DOI: https://doi.org/10.21708/avb.2021.15.1.9363

Mota RA, Silva KPC, Freitas MFL, Porto WJN, Silva LBG. Utilização indiscriminada de antimicrobianos e sua contribuição à multirresistência bacteriana. Braz J Vet Res Anim Sci. 2005;42(6):465-470. DOI: https://doi.org/10.11606/issn.1678-4456.bjvras.2005.26406

Cades M, Silva ZD, Souza HL, Silva JD, Fink AAB, Muniz IM. Perfil de resistência antimicrobiana de mastite bovina em propriedade leiteira no município de Monte Negro/RO. Braz J Sci of Amaz. 2017;6(1):15-20. DOI: https://doi.org/10.47209/2317-5729.v.6.n.1.p.15-20

Naghadi N, Assanzad-Azar H, Delpisheh A. The most important medicinal plants for treatment of brucellosis. J Prev Epidemiol. 2016:543-556.

Sperotto VR, Murari AL, Silva DAR. Brasil. Atividade do decocto de Achyrocline satureioides D.C. (Lam.) - Asteraceae (“macela”) sobre bactérias padrões e isoladas em mastite bovina. Acta Sci Vet. 2012;40(3):1052.

Avancini CAM, Wiest JM. Etnomedicina veterinária, etnosotaxia e Etnoterapêutica de doenças de pele como referência para seleção e avaliação preliminar da atividade antibacteriana de plantas nativas do Sul do Brasil. Rev Bras de Plantas Medicinais. 2008;10:70-78.

Ostrosky EA, Mizumoto MK, Lima MEL, Kaneko TM, Nishikawa SO, Freitas BR. Métodos para avaliação da atividade antimicrobiana e determinação da concentração mínima inibitória (CMI) de plantas medicinais. Rev Bras Farmacogn. 2008;18(2):301-307. DOI: https://doi.org/10.1590/S0102-695X2008000200026

Baracho NC, Oliveira HC, Magalhães IN, Gil BL, Irulegui RSC. Extrato Hidroalcoólico de própolis e cicatrização de feridas no diabetes tipo I: Estudo experimental. Rev Cient Univ. 2019;2:2.

Júnior CC, Ming LC, Scheffer MC. Cultivo de Plantas Medicinais, Condimentares e Aromáticas; 2a. ed., FUNEP, Jaboticabal, São Paulo, 1994.

Matos FJA, Lorenzi H. Plantas medicinais no Brasil, nativas e exóticas. Nova Odessa, Instituto Plantarum de Estudos da Flora LTDA. 512p. 2002.

CLSI. Performance standards for antimicrobial susceptibility testing. 15th Informational Supplement. Document M100-S15. Clinical and Laboratory Standards Institute, Pennsylvania, USA, 2005.

Maciel MJ, Silva MAS, Ethur E, Avancin CAM. Phytochemical indicators and antibacterial activity of the crude hydroalcoholic extract of Achyrocline satureioides ("macela") against antibiotic-resistant Salmonella spp. isolated from animal products (swine and poultry). Rev Bras Hig Sanid Anim. 2017;11:273-287. DOI: https://doi.org/10.5935/1981-2965.20170028

Silva FC, Duarte LP, Filho SAV. Celastráceas: Fontes de Triterpenos Pentacíclicos com Potencial Atividade Biológica. Rev. Virtual Quim. 2014;6(5):1205-1220.

Sá MEP, Melo CB. Disseminação de enfermidades animais por meio do comércio internacional e o papel dos serviços veterinários de fronteira no Brasil. Braz J Vet Med. 2016;38(1):7-12.

Published

2022-07-05

How to Cite

1.
Vasconcelos AB de, Andrade V de, França DA de, Dantas AA, Silva TBC da, Silva KPC da. IN VITRO INHIBITION OF Salmonella spp. ISOLATED FROM CALVES OF THE SERTÃO OF ALAGOAS WITH ETHANOLIC EXTRACTS OF PLANTS FROM THE MATA ATLANTICA. RVZ [Internet]. 2022 Jul. 5 [cited 2024 Nov. 21];29:1-7. Available from: https://rvz.emnuvens.com.br/rvz/article/view/788

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