SENSIBILIDADE DE BACTÉRIAS CAUSADORAS DE MASTITE BOVINA A EXTRATOS DE PLANTAS NATIVAS DO CERRADO
DOI:
https://doi.org/10.35172/rvz.2023.v30.1062Palavras-chave:
fitoterápicos, resistência antimicrobiana, terapêutica veterináriaResumo
A mastite bovina é a doença mais onerosa da produção leiteira e é caracterizada pela inflamação da glândula mamária. O tratamento da doença, sem o controle adequado, gera microrganismos resistentes. Desta forma o uso de fitoterápicos tem se tornado uma fonte de pesquisa como possível alternativa, como as plantas nativas do cerrado Lafoensia pacari, Hymenaea sp. e Stryphnodendron adstringens. No presente trabalho, bactérias isoladas de vacas com mastite subclínica foram identificadas através de testes bioquímicos. Posteriormente foram feitos testes de antibiograma e pesquisa por genes de resistência a antibióticos, por fim foi realizado o teste para avaliação da sensibilidade aos extratos das plantas do cerrado. Foram identificados Staphylococcus aureus (S.aureus), Enterobacter sp. e Escherichia coli (E. coli). Entre os isolados de S. aureus, foram encontrados resistentes a meticilina (MARS), bem como resistentes à vancomicina (VARS). Foram encontrados isolados produtores de beta-lactamases de espectro extendido (ESBL) para Entreobacter sp. e E. coli. Os extratos, principalmente de Stryphnodendron adstringens e Lafoensia pacari, são uma alternativa para a terapêutica antimicrobiana.
Referências
Ruegg PL. A 100-Year Review: Mastitis detection, management, and prevention. J Dairy sci. 2017; 100(12):10381-10397. https://doi.org/10.3168/jds.2017-13023 DOI: https://doi.org/10.3168/jds.2017-13023
Acosta AC, Silva LBGD, Medeiros ES, Pinheiro-Júnior JW, Mota RA. Mastites em ruminantes no Brasil. Pesqui Vet Bras. 2016;36:565-573. https://doi.org/10.1590/S0100-736X2016000700001 DOI: https://doi.org/10.1590/S0100-736X2016000700001
Silva, KC, Lincopan N. Epidemiologia das betalactamases de espectro estendido no Brasil: impacto clínico e implicações para o agronegócio. J Bras Patol Med. 2012;(48):91-99. https://doi.org/10.1590/S1676-24442012000200004 DOI: https://doi.org/10.1590/S1676-24442012000200004
Orsi H. Diversidade genética e pesquisa de Escherichia coli, produtoras de β-lactamase de espectro estendido (ESBL), isolada de leite de vacas com mastite clínica. Repositório institucional UNESP. 2020. Disponível em: https://repositorio.unesp.br/handle/11449/192305
Ali T, Ali I, Khan NA, Han B, Gao J. The Growing Genetic and Functional Diversity of Extended Spectrum Beta-Lactamases. Biomed Res Int. 2018; 9519718: 1-14. https://doi.org/10.1155/2018/9519718 DOI: https://doi.org/10.1155/2018/9519718
Monecke S, Gavier-Widen D, Mattsson R, Rangstrup-Christensen L, Lazaris A, Coleman DC, Shore AC, Ehricht E. Detection of mecC-Positive Staphylococcus aureus (CC130-MRSA-XI) in Diseased European Hedgehogs (Erinaceus europaeus) in Sweden. Plos One. 2013; 8(6): e66166. https://doi.org/10.1371/journal.pone.0066166 DOI: https://doi.org/10.1371/journal.pone.0066166
Cerqueira ES, Almeida RCC. Staphylococcus aureus resistente à meticilina (MRSA) em alimentos de origem animal: uma revisão sistemática. Rev Inst Adolfo Lutz. 2013; 72(4):268-81. https://periodicos.saude.sp.gov.br/index.php/RIAL/article/view/32928 DOI: https://doi.org/10.18241/0073-98552013721574
Pereira EMR, Gomes RT, Freire NR, Aguiar EG, Brandão MDGL, Santos VR. In vitro antimicrobial activity of Brazilian medicinal plant extracts against pathogenic microorganisms of interest to dentistry. Planta med. 2011; 77(04): 401-404. DOI: 10.1055/s-0030-1250354 DOI: https://doi.org/10.1055/s-0030-1250354
Máthé Á, Silva JCS. Introduction to medicinal and aromatic plants in Brazil. In: Medicinal and Aromatic Plants of South America. Springer, Dordrecht, 2018. p. 47-69. DOI: 10.1007/978-94-024-1552-0_3 DOI: https://doi.org/10.1007/978-94-024-1552-0_3
Vieira DS, Peixoto RM, Costa MM, Freire DP, Silva TMG, Silva TMS. Atividade antimicrobiana in vitro do extrato etanólico bruto da folha da Hymenaea martiana Hayne frente às Staphylococcus spp. e avaliação de seu potencial como desinfetante em cabras. Pesq Vet Bras. 2018;(38): 462-469. https://doi.org/10.1590/1678-5150-PVB-4547 DOI: https://doi.org/10.1590/1678-5150-pvb-4547
EURL-AR (Europian Union Reference Laboratory-Antibiotic Resistance). Protocol for PCR amplification of MECA, MECC (MECALGA251), SPA and PVL recommended by the EURL-AR 2ST Version, September 2012; 1-5. Disponível em: https://www.eurl-ar.eu/CustomerData/Files/Folders/21-protocols/279_pcr-spa-pvl-meca-mecc-sept12.pdf
Bajpai T, Pandey M, Varma M, Bhatambare GS. Prevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital. Avicenna J Med. 2017; 07(01):12–6. DOI: 10.4103/2231-0770.197508 DOI: https://doi.org/10.4103/2231-0770.197508
Woodford N, Fagan EJ, Ellington MJ. Multiplex PCR for rapid detection of genes encoding CTX-M extended-spectrum β- lactamases. J Antimicrob Chemother. 2006;57(1):154-155. https://doi.org/10.1093/jac/dki412 DOI: https://doi.org/10.1093/jac/dki412
Almeida TVD. Fatores de risco para mastite bovina e avaliação fenotípica de resistência antimicrobiana [Dissertação]. [Goiânia]: Universidade Federal De Goiás; 2020. Disponível em: https://repositorio.bc.ufg.br/tede/handle/tede/10485.
Intrakamhaeng M, Komutarin T, Pimpukdee K, Aengwanich W. Incidence of enterotoxin-producing MRSA in bovine Mastite cases, bulk milk thands and processing plants in Thailand. J Anim Vet Adv. 2012;11(5):655–61. DOI: 10.3923/javaa.2012.655.661 DOI: https://doi.org/10.3923/javaa.2012.655.661
Pu WX, Su Y, Li JX, Li CH, Yang ZQ, Deng HP, et al. High incidence of oxacillin-susceptible mecA-positive Staphylococcus aureus (OS-MRSA) associated with bovine mastitis in China. PLoS ONE. 2014;11;9(2). https://doi.org/10.1371/journal.pone.0088134 DOI: https://doi.org/10.1371/journal.pone.0088134
Mendonça AT, Carvalho GA, Inácio MCP, Pereira MA. Avaliação antimicrobiana da eficácia dos extratos hidroalcoólicos da pitangueira e da goiabeira, in vitro, contra Staphylococcus aureus isolados de mastite bovina. Rev Augustus. 2021;27(54):59–77. http://orcid.org/0000-0001-6339-392X DOI: https://doi.org/10.15202/10.15202/1981896.2021v27n54p59
Nader TT, Coppede JS, Taleb-Contini SH, Amaral LA, Pereira AMS. Atividade antibiofilme de substâncias de Croton urucurana em Staphylococcus aureus isolado de mastite bovina. Pesq Vet Bras. 2018;38(9):1713–1719. https://doi.org/10.1590/1678-5150-PVB-5034 DOI: https://doi.org/10.1590/1678-5150-pvb-5034
Nader Filho A, Ferreira LM, do Amaral LA, Rossi OD, Oliveira RP. Sensibilidade antimicrobiana dos Staphylococcus aureus isolados no leite de vacas com mastite. Arq Inst Biol. 2007;74(1):1-4. https://doi.org/10.1590/1808-1657v74p0012007 DOI: https://doi.org/10.1590/1808-1657v74p0012007
Aizawa J, Souza-Filho AF, Guimarães AS, Vasconcelos CG, Brito MAVP, Sellera FP, Cortez A, Heinemann MB. Retrospective multicenter study reveals absence of MRSA-associated bovine mastitis in Brazil (1994 to 2016). J Infect Dev Ctries. 2019;13(06): 581-583. doi:10.3855/jidc.11406 DOI: https://doi.org/10.3855/jidc.11406
World Health Organization. Antimicrobial resistance: global report on surveillance. World Health Organization, 2014. Disponível em: https://apps.who.int/iris/bitstream/handle/10665/112642/?sequence=1
Filioussis G, Kachrimanidou M, Christodoulopoulos G, Kyritsi M, Hadjichristodoulou C, Adamopoulou M, Tzivara A, Kritas SK, Grinberg A. Bovine mastitis caused by a multidrug-resistant, mcr-1-positive (colistin-resistant), extended-spectrum β-lactamase–producing Escherichia coli clone on a Greek dairy farm. J Dairy Sci. 2020;103(1):852-857. https://doi.org/10.3168/jds.2019-17320 DOI: https://doi.org/10.3168/jds.2019-17320
Dahmen S, Métayer V, Gay E, Madec JY, Haenni M. Characterization of extended-spectrum beta-lactamase (ESBL)-carrying plasmids and clones of Enterobacteriaceae causing cattle mastitis in France. Vet Microbiol. 2013;162(2-4): 793-799. https://doi.org/10.1016/j.vetmic.2012.10.015 DOI: https://doi.org/10.1016/j.vetmic.2012.10.015
Feng Y, Zhang SD, Shang XF, Wang XR, Ling W, Yan ZT, Li HS. Prevalence and characteristics of extended spectrum β-lactamase-producing Escherichia coli from bovine mastitis cases in China. J Integr Agric. 2018; 17(6):1246-1251. https://doi.org/10.1016/S2095-3119(17)61830-6 DOI: https://doi.org/10.1016/S2095-3119(17)61830-6
Ohnishi M, Okatani AT, Harada K, Sawada T, Marumo K, Murakami M, Sato R, Esaki H, Shimura K, Kato H, Uchida N, Takahashi T. Genetic characteristics of CTX-M-type extended-spectrum-β-lactamase (ESBL)-producing Enterobacteriaceae involved in mastitis cases on Japanese dairy farms, 2007 to 2011. J Clin Microbiol. 2013; 51(9): 3117-3122. DOI: https://doi.org/10.1128/JCM.00920-13 DOI: https://doi.org/10.1128/JCM.00920-13
Karkaba A, Grinberg A, Benschop J, Pleydell E. Characterisation of extended-spectrum β-lactamase and AmpC β-lactamase-producing Enterobacteriaceae isolated from companion animals in New Zealand. N Z Vet J. 2017; 65:2, 105-112. DOI: 10.1080/00480169.2016.1271730 DOI: https://doi.org/10.1080/00480169.2016.1271730
Li L, Wang B, Feng S, Li J, Wu C, Wang Y, Ruan X, Zeng M. Prevalence and characteristics of extended-spectrum β-lactamase and plasmid-mediated fluoroquinolone resistance genes in Escherichia coli isolated from chickens in Anhui province, China. PLoS One, 2014;9, e104356. https://doi.org/10.1371/journal.pone.0104356 DOI: https://doi.org/10.1371/journal.pone.0104356
Cheng J, Qu W, Barkema HW, Nobrega DB, Gao J, Liu G, De Buck G, Kastelic JP, Sun H, Han B. Antimicrobial resistance profiles of 5 common bovine mastitis pathogens in large Chinese dairy herds. J Dairy Sci. 2019; 102(3): 2416-2426. https://doi.org/10.3168/jds.2018-15135 DOI: https://doi.org/10.3168/jds.2018-15135
Timofte D, Maciuca IE, Evans NJ, Williams H, Wattret A, Fick JC, Williams NJ. Detection and molecular characterization of Escherichia coli CTX-M-15 and Klebsiella pneumoniae SHV-12 β-lactamases from bovine mastitis isolates in the United Kingdom. Antimicrob Agents Chemother. 2014;58(2):789-794. https://doi.org/10.1128/AAC.00752-13 DOI: https://doi.org/10.1128/AAC.00752-13
Mubarack HM, Doss A, Dhanabalan R, Venkataswamy R. Activity of some selected medicinal plant extracts against bovine mastitis pathogens. J Anim Vet Adv. 2011;10(6): 738-741. DOI: 10.3923/javaa.2011.738.741 DOI: https://doi.org/10.3923/javaa.2011.738.741
Lopes TS, Fontoura PS, Oliveira A, Rizzo FA, Silveira S, Streck AF. Use of plant extracts and essential oils in the control of bovine mastitis. Res Vet Sci. 2020;131:186-193. https://doi.org/10.1016/j.rvsc.2020.04.025 DOI: https://doi.org/10.1016/j.rvsc.2020.04.025
Mushtaq S, Shah AM, Shah A, Lone SA, Hussain A, Hassan QP, Ali MN. Bovine mastitis: An appraisal of its alternative herbal cure. Microb Pathog. 2018;114:357-361. https://doi.org/10.1016/j.micpath.2017.12.024 DOI: https://doi.org/10.1016/j.micpath.2017.12.024
Porfírio Z, Melo-Filho GC, Alvino V, Lima MRF, Sant'Ana AE. Atividade antimicrobiana de extratos hidroalcoólicos de Lafoensia pacari A. St.-Hil., Lythraceae, frente a bactérias multirresistentes de origem hospitalar. Rev Bras Farmacogn. 2009; 19:785-789. https://doi.org/10.1590/S0102-695X2009000500023 DOI: https://doi.org/10.1590/S0102-695X2009000500023
Kovačević Z, Radinović M, Čabarkapa I, Kladar N, Božin B. Natural agents against bovine mastitis pathogens. Antibiotics. 2021;10(2):205. https://doi.org/10.3390/antibiotics10020205 DOI: https://doi.org/10.3390/antibiotics10020205
Chen X, Shang F, Meng Y, Li L, Cui Y, Zhang M, Qi K, Xue T. Ethanol extract of Sanguisorba officinalis L. inhibits biofilm formation of methicillin-resistant Staphylococcus aureus in an ica-dependent manner. J Dairy Sci. 2015;98(12):8486-8491. https://doi.org/10.3168/jds.2015-9899 DOI: https://doi.org/10.3168/jds.2015-9899
Tegos G, Stermitz FR, Lomovskaya O, Lewis K. Multidrug pump inhibitors uncover remarkable activity of plant antimicrobials. Antimicrob Agents Chemother. 2002;46(10):3133-3141. DOI: https://doi.org/10.1128/AAC.46.10.3133-3141.2002 DOI: https://doi.org/10.1128/AAC.46.10.3133-3141.2002
Fratini F, Casella S, Leonardi M, Pisseri F, Ebani VV, Pistelli L, Pistelli L. Antibacterial activity of essential oils, their blends and mixtures of their main constituents against some strains supporting livestock mastitis. Fitoterapia. 2014; 96:1-7. https://doi.org/10.1016/j.fitote.2014.04.003 DOI: https://doi.org/10.1016/j.fitote.2014.04.003
Ribeiro ICO, Mariano EGA, Careli RT, Morais-Costa F, Sant’Anna FM, Pinto MS, Souza MR, Duarte ER. Plants of the Cerrado with antimicrobial effects against Staphylococcus spp. and Escherichia coli from cattle. BMC Vet Res. 2018; 14(1):1-10. DOI 10.1186/s12917-018-1351-1 DOI: https://doi.org/10.1186/s12917-018-1351-1
Downloads
Publicado
Como Citar
Edição
Seção
Licença
Este obra está licenciado com uma Licença Creative Commons Atribuição-NãoComercial 4.0 Internacional.