Expressão gênica

Uma visão geral dos métodos e aplicações na pesquisa do câncer

  • Marina Mitie de Souza Monobe
  • Rodrigo Costa da Silva
Palavras-chave: sequência gênica, expressão gênica, técnicas moleculares, genótipo, oncologia veterinária

Resumo

A expressão genética é o estudo de como o genótipo dá origem ao fenótipo a partir da
investigação da quantidade de RNAm transcrito em um sistema biológico. Vários métodos já
foram padronizados para identificar variações na expressão gênica, dentre eles a hibridização
subtrativa, “differential display”, análise em série da expressão genética, hibridização de
microarranjo, e sequenciamento por RNA-seq. A maioria das técnicas tem focado na pesquisa
e diagnóstico do câncer, gerando enorme quantidade de dados, o que permitiu compreender a
progressão do câncer e suas vias, descobrir e analisar novas intervenções terapêuticas, novas
ferramentas moleculares para o diagnóstico e prognóstico, e analisar o tempo de
sobrevivência em pacientes humanos e animais. Desta forma, as diferentes técnicas de
expressão gênica trouxeram novas e importantes perspectivas para a área médica e veterinária,
e novas pesquisas focadas em oncologia fornecerão muito mais conhecimento sobre as vias e
interações entre células saudáveis e tumorais, melhorando as perspectivas das intervenções
diárias pelos oncologistas e clínicos.

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Publicado
20-12-2016
Como Citar
Mitie de Souza Monobe, M., & Costa da Silva, R. (2016). Expressão gênica: Uma visão geral dos métodos e aplicações na pesquisa do câncer. Veterinária E Zootecnia, 23(4), 532-546. Recuperado de https://rvz.emnuvens.com.br/rvz/article/view/406
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