BODY REGION INFLUENCE ON TUBERCULOSIS IMMUNE ALLERGIC DIAGNOSIS IN GOATS

Abstract

Allergic immune response was evaluated applying bovine purified protein derivative (PPD), intradermally injected on different body regions surface of goats (Capra hircus) experimentally sensitized to Mycobacterium bovis strain AN₅. It was used 20 clinically healthy adult goats, of which 14 animals constituted the experimental group, receiving the bovine PPD in different body regions (cranial cervical, caudal cervical, ventral thoracic, dorsal thoracic, ventral abdominal, dorsal abdominal and tail fold), and six animals were inoculated with saline as control group. The goats were monitored by clinical examinations and PPD’s measurements made with skin fold calipers, 24h before, 0h, 24h, 48h, 72h and 96 hours after application. The results obtained showed that the highest increases of skin thickness were found on the abdominal dorsal, thoracic dorsal and caudal cervical regions at 24h, 48h and 72 hours of application. In the tail fold and the ventral thoracic and abdominal regions, increases of skin thickness were lower than observed on other regions (p <0.05). The analysis of all results recommend that the simple allergic immune test for tuberculosis in goats, should be measured after 72 hours of application and PPD should be intradermally injected predominantly in dorsal abdominal, thoracic dorsal or caudal cervical regions.