LISTERIA MONOCYTOGENES IN COW’S MILK PRODUCED BY FAMILY-OWNED DAIRY FARMS
DOI:
https://doi.org/10.35172/rvz.2020.v27.674Keywords:
raw milk, listeriosis, microbiology, molecular biology, diagnosis.Abstract
Refrigeration is an important milk preservation method. However, milk quality may deteriorate if the product is refrigerated for long periods, mainly due to the growth of psychrotrophic bacteria. This group of microorganisms includes pathogenic genera, most notably Listeria monocytogenes. The detection of this bacterium in food is important, given its pathogenic effects on human and animal health and also its economic relevance. This study focused on detecting the presence of L. monocytogenes in milk samples collected at small family-owned dairy farms. Samples were cultivated on PALCAM and ALOA agars for microbiological analysis and a molecular analysis by polymerase chain reaction was performed for the detection of L. monocytogenes. Despite the negative results obtained in both these analyses, further studies are recommended to confirm or refute the negligible effect of L. monocytogenes on small dairy farms.
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