Leishmania infantum (syn. chagasi) infection in cats from a Brazilian southeastern endemic area for canine and human leishmaniasis
Keywords:
cats, leishmaniasis, IFAT, hemoculture, PCRAbstract
Visceral leishmaniasis (VL) is an important zoonosis caused by Leishmania infantum (syn.
chagasi) in Americas and transmitted by Lutzomyia longipalpis, genus phlebotomines. The
diagnosis of Leishmania infection in cats is very important to better comprehend the role of
felines on LV’s epidemiological chain. This study aimed to determine the occurrence of L.
infantum (syn. chagasi) in 109 cats from Birigui, SP, Brazil, an endemic area for canine and
human leishmaniasis, by using Indirect Fluorescent Antibody Test (IFAT), hemoculture and
Polymerase Chain Reaction (PCR). Fifty two (47.7%; CI95% 38.6-57.0%) samples were
positive by the hemoculture, three (2.8%; CI95% 1.0-7.8%) by IFAT and 17 (15.6%; CI95%
10.0-23.6%) by PCR. According to the results, we can conclude that cats from endemic areas
for the infection can be frequently exposed to the agent and suggest the importance of these
animals in the epidemiological cycle, demonstrating the need for further research and
diagnostic alternatives to aid in the establishment of preventive steps to control this disease.
References
WHO. World Health Organization. Control of the Leishmaniasis: Report of a meeting of the WHO Expert Committee on the Control of Leishmaniases. 2010. Geneva, 22-26 March 2010. Report n. 949.
Simões-Mattos L, Teixeira MJ, Costa DC, Prata-JR JRC, Bevilaqua CML, Sidrim JJC. et al. Evaluation of terbinafine treatment in Leishmania chagasi-infected hamsters (Mesocricetus auratus). Vet Parasitol. 2002; 103:207-216.
Da Silva AVM, Cândido CDS, Pereira DP, Brazil RP, Carreira JCA. The first Record of American visceral leishmaniasis in domestic cats from Rio de Janeiro, Brazil. Acta Tropica. 2008; 105:92-94.
Gontijo CMF, Melo MN. Leishmaniose visceral no Brasil: quadro atual, desafios e perspectivas. Rev Bras Epidemiol. 2004;7:338-349.
Simões-Mattos L, Mattos MRF, Teixeira MJ, Oliveira-Lima JW, Bevilaqua CML, Prata-Júnior RC. et al. The susceptibility of domestic cats (Felis catus) to experimental infection with Leishmania braziliensis. Vet Parasitol. 2005; 127:199-208.
Coelho WMD, Lima VMF, Soutel, RVG, Amarante AFT, Gennari SM, Meireles MV, et al. Ocorrência de anticorpos contra Leishmania spp. em gatos domésticos do município de Andradina-SP-Brasil. Vet Zootec. 2008; 15:75.
Ikonomopoulos J, Kokotas S, Gazouli M, Zavras A, Stoitsiou M, Gorgoulis VG. Molecular diagnosis of leishmaniosis in dogs. Comparative application of traditional diagnostic methods and the proposed assay on clinical samples. Vet Parasitol. 2003; 113:99-103.
SINAN. Sistema de Informação de Agravos de Notificação. Leishmaniose visceral - casos notificados confirmados no Sinan. Available in: http://dtr2004.saude.gov.br/sinanweb/tabnet/tabnet?sinannet/leishvi/bases/leishvbrnet.def. Accessed in 5 mar. 2016.
Luz ZMP. Changes in the Hemoculture Methodology Improve the Test Positivity. Mem Inst Oswaldo Cruz. 1999; 94:295-298.
Camargo ME. Introdução às técnicas de imunofluorescência. Rev Bras Patol Clin.1974; 10(4):143-171.
Brasil Manual de Vigilância e Controle da Leishmaniose Visceral. Normas e Manuais Técnicos, Séria A, Departamento de Vigilância Epidemiológica, Secretaria de Vigilância em Saúde, Ministério da Saúde, Brasília. 120p. 2006.
Rosypal AC, Cortés-Vecino JA, Gennari SM, Dubey JP, Tidwell RR, Lindsay DS. Serological survey of Leishmania infantum and Trypanosoma cruzi in dogs from urban areas of Brazil and Colombia. Vet Parasitol. 2007; 149:172-177.
Figueiredo FB, Bonna IC, Nascimento LD, Costa T, Baptista C, Pacheco TM, et al. Serological evaluation for detection of anti-Leishmania antibodies in dogs and cats in the district of Santa Rita de Cássia, municipality of Barra Mansa, State of Rio de Janeiro. Rev Soc Bras Med Trop. 2009; 42:141-145.
Lachaud L, Marchergui-Hammami S, Chabbert E, Dereure J, Dedet JP, Bastien P. Comparision of six PCR methods using peripheral blood for detection of canine visceral leishmaniasis. J Clin Microbiol. 2002; 40:210-215.
Triola MF. 2005. Introdução à estatística. 9.ed. Rio de Janeiro: LTC. 682p.
Mackinnon DP. 2000. Introduction to statistical mediation analysis. New York: Routledge Academic. 488p.
Passos VMA, Lasmar EB, Gontijo CMF, Fernandes O, Degrave W. Natural infection of a domestic cat (Felis domesticus) with Leishmania (Viannia) in the metropolitan region of Belo Horizonte, State of Minas Gerais, Brazil. Mem Inst Oswaldo Cruz. 1996; 91(1): 19-20.
Savani ES, Camargo MCO, Carvalho MR, Zampieri RA, Santos MG, D'Auria SR, et al. The first record in the Americas of an autochthonous case of Leishmania (Leishmania) infantum chagasi in a domestic cat (Felix catus) from Cotia County, São Paulo State, Brazil. Vet Parasitol. 2004; 120:229-233.
Souza AI, Barros EMS, Ishikawa E, Ilha IMN, Marin GRB, Sundar S, et al. Laboratory diagnosis of visceral leishmaniasis. Clin Diag Lab Immunol. 2002; 9:951-958.
Chiari E. Chagas disease diagnosis using polymerase chain reaction, hemoculture and serologic methods. Mem Inst Oswaldo Cruz. 1999; 94:299-300.
De Carli GA. Parasitologia Clínica: Seleção de Métodos e Técnicas de Laboratório para o Diagnóstico de Parasitoses Humanas. Atheneu: Rio de Janeiro, 810p. 2001.
Junqueira ACV, Chiari E, Wincker P. Comparison of the polymerase chain reaction with two classical parasitological methods for the diagnosis of Chagas disease in an endemic region of North-eastern Brazil. Trans Royal Soc Trop Med Hyg. 1996; 90:129-132.
Luciano RM, Lucheis SB, Troncarelli MZ, Luciano DM, Langoni H. Avaliação da reatividade cruzada entre antígenos de Leishmania spp. e Trypanosoma cruzi na resposta sorológica de cães pela técnica de Imunofluorescência Indireta (RIFI). Braz J Vet Res Anim Sci. 2009; 46:181-187.
Braga ARC. Hemocultura, reação de imunofluorescência indireta (RIFI) e reação em cadeia pela polimerase para Leishmania spp. em cães e gatos provenientes de área endêmica e não endêmica para leishmaniose. Botucatu, Dissertação de Mestrado, Faculdade de Medicina Veterinária e Zootecnia, Campus de Botucatu, Universidade Estadual Paulista, Botucatu, SP. 100p. 2009.
Poli A, Abramo F, Barsotti P, Leva S, Gramiccia M, Ludovisi A, et al. Feline leishmaniosis due to Leishmania infantum in Italy. Vet Parasitol. 2002; 106:181-191.
Oliveira ALL, Paniago AMM, Dorval MCE, Oshiro ET, Leal CR, Sanches M. Emergent outbreak of visceral leishmaniasis in Mato Grosso do Sul State. Rev Soc Bras Med Trop. 2002; 39:446-450.
Maroli M, Penissi MG, Muccio T, Khoury C, Gradoni L, Gramiccia M. Infection of sandflies by a cat naturally infected with Leishmania infantum. Vet Parasitol. 2007; 145:357-360.
Martín-Sanchez J, Acedo C, Muñoz-Pérez M, Pesson B, Marchal O, Morillas-Márquez F. Infection by Leishmania infantum in cats: Epidemiological study in Spain. Vet Parasitol. 2007; 145:267-273.
Maia C, Nunes M, Campino L. Importance of cats in zoonotic leishmaniasis in Portugal. Vector Borne Zoonotic Dis. 2008; 8:555-559.
Ayllon T, Tesouro MA, Amusategui I, Villaescusa A, Rodrigues-Franco F, Sainz A. Serologic and molecular evaluation of Leishmania infantum in cats from Central Spain. Ann NY Acad Sci. 2008; 1149:361-364.
Beard CB, Pye G, Steurer FJ., Rodriguez R., Campman R, Peterson AT, et al. Chagas disease in a domestic transmission cycle, southerm Texas, USA. Emerg Infect Dis. 2003; 9(1):103-105.
Silveira TGV, Teodoro U, Lonardoni MVC, Toledo MJD, Bertolini DA, Arraes SMAA, et al. Investigação sorológica em cães de área endêmica de leishmaniose tegumentar no Estado do Paraná, Sul do Brasil. Cad Saúde Pública. 1996; 12:89-93.
Costa TAC, Rossi CN, Laurenti MD, Gomes AAD, Vides JP, Sobrinho LSV, et al. Ocorrência de leishmaniose em gatos de áreas endêmicas para leishmaniose visceral. Braz J Vet Res Anim Sci. 2010; 47:213-217.
Dye C, Vidor E, Dereure J. Serological diagnosis of leishmaniasis: on detecting infection as well as disease. Epidemiol Infect. 1993; 103:647-656.
Maia C, Gomes J, Cristóvão J, Nunes M, Martins A, Rebêlo E, et al. Feline Leishmania infection in a canine leishmaniasis endemic region, Portugal. Vet Parasitol. 2010; 174:336-340.
Portús M, Gállego M, Riera C, Aisa M, Fisa R, Castillejo S. Wild and domestic mammals in the life cycle of Leishmania infantum in Southwest Europe. A literature review and studies performed in Catalonia (Spain). Rev Iberica Parasitol. 2002; 62:72.
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